Common-path multimodal optical microscopy

Chandra S. Yelleswarapu, Marla Tipping, Sri-Rajasekhar Kothapalli, Alexey Veraksa, D. V.G.L.N. Rao

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have developed a common-path multimodal optical microscopy system that is capable of using a single optical source and a single camera to image amplitude, phase, and fluorescence features of a biological specimen. This is achieved by varying either contrast enhancement filters at the Fourier plane and/or neutral density/fluorescence filters in front of the CCD camera. The feasibility of the technique is demonstrated by obtaining brightfield, fluorescence, phase-contrast, spatially filtered, brightfield + fluorescence, phase + fluorescence, and edge-enhanced+fluorescence images of the same Drosophila embryo without the need for image registration and fusion. This comprehensive microscope has the capability of providing both structural and functional information and may be used for applications such as studying live-cell dynamics and in high throughput microscopy and automated microscopy.

Original languageEnglish (US)
Pages (from-to)1243-1245
Number of pages3
JournalOptics Letters
Volume34
Issue number8
DOIs
StatePublished - Apr 15 2009

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optical paths
microscopy
fluorescence
Drosophila
filters
embryos
phase contrast
CCD cameras
fusion
cameras
microscopes
augmentation
cells

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

Cite this

Yelleswarapu, C. S., Tipping, M., Kothapalli, S-R., Veraksa, A., & Rao, D. V. G. L. N. (2009). Common-path multimodal optical microscopy. Optics Letters, 34(8), 1243-1245. https://doi.org/10.1364/OL.34.001243
Yelleswarapu, Chandra S. ; Tipping, Marla ; Kothapalli, Sri-Rajasekhar ; Veraksa, Alexey ; Rao, D. V.G.L.N. / Common-path multimodal optical microscopy. In: Optics Letters. 2009 ; Vol. 34, No. 8. pp. 1243-1245.
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Yelleswarapu, CS, Tipping, M, Kothapalli, S-R, Veraksa, A & Rao, DVGLN 2009, 'Common-path multimodal optical microscopy', Optics Letters, vol. 34, no. 8, pp. 1243-1245. https://doi.org/10.1364/OL.34.001243

Common-path multimodal optical microscopy. / Yelleswarapu, Chandra S.; Tipping, Marla; Kothapalli, Sri-Rajasekhar; Veraksa, Alexey; Rao, D. V.G.L.N.

In: Optics Letters, Vol. 34, No. 8, 15.04.2009, p. 1243-1245.

Research output: Contribution to journalArticle

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Yelleswarapu CS, Tipping M, Kothapalli S-R, Veraksa A, Rao DVGLN. Common-path multimodal optical microscopy. Optics Letters. 2009 Apr 15;34(8):1243-1245. https://doi.org/10.1364/OL.34.001243