Community fingerprinting of dominant populations in hydrogen-producing bioreactors

Maryann Victoria Bruns, Yonghua Luo, Husen Zhang, Steve Van Ginkel, Bruce Ernest Logan

Research output: Contribution to journalConference articlepeer-review


Biological H2 production in engineered reactors was studied and the microbial communities responsible by molecular biology techniques were characterized. Ribosomal intergenic spacer analysis (RISA) fingerprints were obtained from bacterial communities in continuous-flow bioreactors inoculated with heat-treated soil and operated at 30° and 37°C. DNA bands from fingerprints consistently yielded Clostridia spp. belonging to Cluster I (Stackebrandt and Hippe, 2001). Subcluster Ib populations were closely related to the solventogenic Clostridium acetobutylicum and Ic populations to C. acidisoli, an acid-tolerant isolate from forest litter. Using subsamples of the same heat-treated soil inoculum, independent reactor trials exhibited different N2 production rates and efficiencies when glucose concentrations were from 1-10 g/L. Only populations belonging to Clostridiaceae were detected in all RISA fingerprints. Clostridium acidisoli relatives (Cluster Ic clostridia} were detected with greatest frequency at lower glucose concentrations, while Cluster Ib clostridia were most frequently detected at higher glucose concentrations. This is an abstract of a paper presented at the 228th ACS National Meeting (Philadelphia, PA 8/22-26/2004).

Original languageEnglish (US)
Pages (from-to)1558-1560
Number of pages3
JournalACS, Division of Environmental Chemistry - Preprints of Extended Abstracts
Issue number2
StatePublished - Dec 1 2004
Event228th ACS National Meeting - Philadelphia, PA, United States
Duration: Aug 22 2004Aug 26 2004

All Science Journal Classification (ASJC) codes

  • Energy(all)


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