Comparative ability of chicken and mammalian LHRH to release LH from rooster pituitary cells in vitro

Alan Leslie Johnson, R. W. Dickerman, J. P. Advis

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Biological properties of homogeneous solutions of chicken (c) and mammalian (m) LHRH were compared by their ability to release LH, in vitro, from a rooster pituitary cell incubation system. Homogeneity of the two LHRH species was confirmed by High Performance Liquid Chromatography (HPLC) using linear gradients of acetonitrile and phosphate buffer. A clear HPLC separation of [Gln8]-LHRH (cLHRH) and [Arg8]-LHRH (mLHRH) was obtained, with the former having a consistantly longer retention time than the latter. cLHRH cause a greater (p<.025) in vitro release of LH at low doses (<1 ng/2×105 live pituitary cells), but not at high doses (>10 ng/2×105 live pituitary cells), than that caused by mLHRH. Our results indicate that rooster pituitary cells are significantly more sensitive to low doses of cLHRH than to similar doses of mLHRH, when assesed by their ability to release LH in vitro.

Original languageEnglish (US)
Pages (from-to)1847-1851
Number of pages5
JournalLife Sciences
Volume34
Issue number19
DOIs
StatePublished - May 7 1984

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Gonadotropin-Releasing Hormone
Chickens
High performance liquid chromatography
High Pressure Liquid Chromatography
Buffers
Phosphates
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Johnson, Alan Leslie ; Dickerman, R. W. ; Advis, J. P. / Comparative ability of chicken and mammalian LHRH to release LH from rooster pituitary cells in vitro. In: Life Sciences. 1984 ; Vol. 34, No. 19. pp. 1847-1851.
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Comparative ability of chicken and mammalian LHRH to release LH from rooster pituitary cells in vitro. / Johnson, Alan Leslie; Dickerman, R. W.; Advis, J. P.

In: Life Sciences, Vol. 34, No. 19, 07.05.1984, p. 1847-1851.

Research output: Contribution to journalArticle

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AU - Dickerman, R. W.

AU - Advis, J. P.

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AB - Biological properties of homogeneous solutions of chicken (c) and mammalian (m) LHRH were compared by their ability to release LH, in vitro, from a rooster pituitary cell incubation system. Homogeneity of the two LHRH species was confirmed by High Performance Liquid Chromatography (HPLC) using linear gradients of acetonitrile and phosphate buffer. A clear HPLC separation of [Gln8]-LHRH (cLHRH) and [Arg8]-LHRH (mLHRH) was obtained, with the former having a consistantly longer retention time than the latter. cLHRH cause a greater (p<.025) in vitro release of LH at low doses (<1 ng/2×105 live pituitary cells), but not at high doses (>10 ng/2×105 live pituitary cells), than that caused by mLHRH. Our results indicate that rooster pituitary cells are significantly more sensitive to low doses of cLHRH than to similar doses of mLHRH, when assesed by their ability to release LH in vitro.

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