Comparative analysis of the α-like globin clusters in mouse, rat, and human chromosomes indicates a mechanism underlying breaks in conserved synteny

Cristina Tufarelli, Ross Cameron Hardison, Webb Miller, Jim Hughes, Kevin Clark, Nicki Ventress, Anna Maria Frischauf, Douglas R. Higgs

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We have sequenced and fully annotated a 65,871-bp region of mouse Chromosome 17 including the Hba-ps4 α-globin pseudogene. Comparative sequence analysis with the functional α-globin loci at human Chromosome 16p13.3 and mouse Chromosome 11 shows that this segment of mouse Chromosome 17 contains a group of three α-like pseudogenes (Hba-psm-Hba-ps4-Hba-q3), similar to the duplicated sets found at the functional mouse cluster on Chromosome 11. In addition, exons 7 to 12 of the mLuc7L gene are present just downstream from the pseudogene cluster, indicating that this clone contains the region in which human 16p13.3 switches in synteny between mouse Chromosomes 11 and 17. Comparison of the sequences around the α-like clusters on the two mouse chromosomes reveals the presence of conserved tandem repeats. We propose that these repetitive elements have played a role in the fragmentation of the mouse α cluster during evolution.

Original languageEnglish (US)
Pages (from-to)623-630
Number of pages8
JournalGenome research
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2004

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Synteny
Globins
Human Chromosomes
Chromosomes, Human, Pair 11
Chromosomes, Human, Pair 17
Pseudogenes
Tandem Repeat Sequences
Sequence Analysis
Exons
Clone Cells
Chromosomes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

Tufarelli, Cristina ; Hardison, Ross Cameron ; Miller, Webb ; Hughes, Jim ; Clark, Kevin ; Ventress, Nicki ; Frischauf, Anna Maria ; Higgs, Douglas R. / Comparative analysis of the α-like globin clusters in mouse, rat, and human chromosomes indicates a mechanism underlying breaks in conserved synteny. In: Genome research. 2004 ; Vol. 14, No. 4. pp. 623-630.
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Comparative analysis of the α-like globin clusters in mouse, rat, and human chromosomes indicates a mechanism underlying breaks in conserved synteny. / Tufarelli, Cristina; Hardison, Ross Cameron; Miller, Webb; Hughes, Jim; Clark, Kevin; Ventress, Nicki; Frischauf, Anna Maria; Higgs, Douglas R.

In: Genome research, Vol. 14, No. 4, 01.04.2004, p. 623-630.

Research output: Contribution to journalArticle

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