Comparative evaluation of AmpliVue HSV 1+2 assay with ELVIS culture for detecting herpes simplex Virus 1 (HSV-1) and HSV-2 in clinical specimens

Paul A. Granato, Brenda R. Alkins, Belinda Yen-Lieberman, Wallace H. Greene, Jessica Connolly, Blake W. Buchan, Nathan A. Ledeboer

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The AmpliVue HSV 1+2 assay was compared to the ELVIS HSV ID and D3 Typing Culture System for the qualitative detection and differentiation of herpes simplex virus 1 (HSV-1) and HSV-2 DNA in 1,351 cutaneous and mucocutaneous specimens. Compared to ELVIS, AmpliVue had sensitivities of 95.7 and 97.6% for detecting HSV-1 and HSV-2, respectively. Following arbitration of discordant results by an independent molecular method, the AmpliVue assay had a resolved sensitivity and specificity of 99.2 and 99.7%, respectively, for both HSV-1 and HSV-2, whereas ELVIS had a resolved sensitivity of 87.1% for HSV-1 and 84.5% for HSV-2.

Original languageEnglish (US)
Pages (from-to)3922-3925
Number of pages4
JournalJournal of clinical microbiology
Issue number12
Publication statusPublished - Dec 2015


All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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