Comparative evaluation of AmpliVue HSV 1+2 assay with ELVIS culture for detecting herpes simplex Virus 1 (HSV-1) and HSV-2 in clinical specimens

Paul A. Granato, Brenda R. Alkins, Belinda Yen-Lieberman, Wallace H. Greene, Jessica Connolly, Blake W. Buchan, Nathan A. Ledeboer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The AmpliVue HSV 1+2 assay was compared to the ELVIS HSV ID and D3 Typing Culture System for the qualitative detection and differentiation of herpes simplex virus 1 (HSV-1) and HSV-2 DNA in 1,351 cutaneous and mucocutaneous specimens. Compared to ELVIS, AmpliVue had sensitivities of 95.7 and 97.6% for detecting HSV-1 and HSV-2, respectively. Following arbitration of discordant results by an independent molecular method, the AmpliVue assay had a resolved sensitivity and specificity of 99.2 and 99.7%, respectively, for both HSV-1 and HSV-2, whereas ELVIS had a resolved sensitivity of 87.1% for HSV-1 and 84.5% for HSV-2.

Original languageEnglish (US)
Pages (from-to)3922-3925
Number of pages4
JournalJournal of clinical microbiology
Volume53
Issue number12
DOIs
StatePublished - Dec 2015

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Human Herpesvirus 2
Human Herpesvirus 1
Negotiating
glutamyl-leucyl-valyl-isoleucyl-serine
Sensitivity and Specificity
Skin
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Granato, Paul A. ; Alkins, Brenda R. ; Yen-Lieberman, Belinda ; Greene, Wallace H. ; Connolly, Jessica ; Buchan, Blake W. ; Ledeboer, Nathan A. / Comparative evaluation of AmpliVue HSV 1+2 assay with ELVIS culture for detecting herpes simplex Virus 1 (HSV-1) and HSV-2 in clinical specimens. In: Journal of clinical microbiology. 2015 ; Vol. 53, No. 12. pp. 3922-3925.
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abstract = "The AmpliVue HSV 1+2 assay was compared to the ELVIS HSV ID and D3 Typing Culture System for the qualitative detection and differentiation of herpes simplex virus 1 (HSV-1) and HSV-2 DNA in 1,351 cutaneous and mucocutaneous specimens. Compared to ELVIS, AmpliVue had sensitivities of 95.7 and 97.6{\%} for detecting HSV-1 and HSV-2, respectively. Following arbitration of discordant results by an independent molecular method, the AmpliVue assay had a resolved sensitivity and specificity of 99.2 and 99.7{\%}, respectively, for both HSV-1 and HSV-2, whereas ELVIS had a resolved sensitivity of 87.1{\%} for HSV-1 and 84.5{\%} for HSV-2.",
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Comparative evaluation of AmpliVue HSV 1+2 assay with ELVIS culture for detecting herpes simplex Virus 1 (HSV-1) and HSV-2 in clinical specimens. / Granato, Paul A.; Alkins, Brenda R.; Yen-Lieberman, Belinda; Greene, Wallace H.; Connolly, Jessica; Buchan, Blake W.; Ledeboer, Nathan A.

In: Journal of clinical microbiology, Vol. 53, No. 12, 12.2015, p. 3922-3925.

Research output: Contribution to journalArticle

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