Comparative Genomics of Multiple Strains of Pseudomonas cannabina pv. alisalensis, a Potential Model Pathogen of Both Monocots and Dicots

Panagiotis F. Sarris, Emmanouil A. Trantas, David A. Baltrus, Carolee Theresa Bull, William Patrick Wechter, Shuangchun Yan, Filippos Ververidis, Nalvo F. Almeida, Corbin D. Jones, Jeffery L. Dangl, Nickolas J. Panopoulos, Boris A. Vinatzer, Dimitrios E. Goumas

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Comparative genomics of closely related pathogens that differ in host range can provide insights into mechanisms of host-pathogen interactions and host adaptation. Furthermore, sequencing of multiple strains with the same host range reveals information concerning pathogen diversity and the molecular basis of virulence. Here we present a comparative analysis of draft genome sequences for four strains of Pseudomonas cannabina pathovar alisalensis (Pcal), which is pathogenic on a range of monocotyledonous and dicotyledonous plants. These draft genome sequences provide a foundation for understanding host range evolution across the monocot-dicot divide. Like other phytopathogenic pseudomonads, Pcal strains harboured a hrp/hrc gene cluster that codes for a type III secretion system. Phylogenetic analysis based on the hrp/hrc cluster genes/proteins, suggests localized recombination and functional divergence within the hrp/hrc cluster. Despite significant conservation of overall genetic content across Pcal genomes, comparison of type III effector repertoires reinforced previous molecular data suggesting the existence of two distinct lineages within this pathovar. Furthermore, all Pcal strains analyzed harbored two distinct genomic islands predicted to code for type VI secretion systems (T6SSs). While one of these systems was orthologous to known P. syringae T6SSs, the other more closely resembled a T6SS found within P. aeruginosa. In summary, our study provides a foundation to unravel Pcal adaptation to both monocot and dicot hosts and provides genetic insights into the mechanisms underlying pathogenicity.

Original languageEnglish (US)
Article numbere59366
JournalPloS one
Volume8
Issue number3
DOIs
StatePublished - Mar 28 2013

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Pathogens
Genomics
Pseudomonas
Magnoliopsida
Liliopsida
Genes
Host Specificity
genomics
pathogens
host range
Genome
Virulence
Genomic Islands
multigene family
genome
Host-Pathogen Interactions
genomic islands
type III secretion system
Conservation
Multigene Family

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sarris, Panagiotis F. ; Trantas, Emmanouil A. ; Baltrus, David A. ; Bull, Carolee Theresa ; Wechter, William Patrick ; Yan, Shuangchun ; Ververidis, Filippos ; Almeida, Nalvo F. ; Jones, Corbin D. ; Dangl, Jeffery L. ; Panopoulos, Nickolas J. ; Vinatzer, Boris A. ; Goumas, Dimitrios E. / Comparative Genomics of Multiple Strains of Pseudomonas cannabina pv. alisalensis, a Potential Model Pathogen of Both Monocots and Dicots. In: PloS one. 2013 ; Vol. 8, No. 3.
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title = "Comparative Genomics of Multiple Strains of Pseudomonas cannabina pv. alisalensis, a Potential Model Pathogen of Both Monocots and Dicots",
abstract = "Comparative genomics of closely related pathogens that differ in host range can provide insights into mechanisms of host-pathogen interactions and host adaptation. Furthermore, sequencing of multiple strains with the same host range reveals information concerning pathogen diversity and the molecular basis of virulence. Here we present a comparative analysis of draft genome sequences for four strains of Pseudomonas cannabina pathovar alisalensis (Pcal), which is pathogenic on a range of monocotyledonous and dicotyledonous plants. These draft genome sequences provide a foundation for understanding host range evolution across the monocot-dicot divide. Like other phytopathogenic pseudomonads, Pcal strains harboured a hrp/hrc gene cluster that codes for a type III secretion system. Phylogenetic analysis based on the hrp/hrc cluster genes/proteins, suggests localized recombination and functional divergence within the hrp/hrc cluster. Despite significant conservation of overall genetic content across Pcal genomes, comparison of type III effector repertoires reinforced previous molecular data suggesting the existence of two distinct lineages within this pathovar. Furthermore, all Pcal strains analyzed harbored two distinct genomic islands predicted to code for type VI secretion systems (T6SSs). While one of these systems was orthologous to known P. syringae T6SSs, the other more closely resembled a T6SS found within P. aeruginosa. In summary, our study provides a foundation to unravel Pcal adaptation to both monocot and dicot hosts and provides genetic insights into the mechanisms underlying pathogenicity.",
author = "Sarris, {Panagiotis F.} and Trantas, {Emmanouil A.} and Baltrus, {David A.} and Bull, {Carolee Theresa} and Wechter, {William Patrick} and Shuangchun Yan and Filippos Ververidis and Almeida, {Nalvo F.} and Jones, {Corbin D.} and Dangl, {Jeffery L.} and Panopoulos, {Nickolas J.} and Vinatzer, {Boris A.} and Goumas, {Dimitrios E.}",
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Sarris, PF, Trantas, EA, Baltrus, DA, Bull, CT, Wechter, WP, Yan, S, Ververidis, F, Almeida, NF, Jones, CD, Dangl, JL, Panopoulos, NJ, Vinatzer, BA & Goumas, DE 2013, 'Comparative Genomics of Multiple Strains of Pseudomonas cannabina pv. alisalensis, a Potential Model Pathogen of Both Monocots and Dicots', PloS one, vol. 8, no. 3, e59366. https://doi.org/10.1371/journal.pone.0059366

Comparative Genomics of Multiple Strains of Pseudomonas cannabina pv. alisalensis, a Potential Model Pathogen of Both Monocots and Dicots. / Sarris, Panagiotis F.; Trantas, Emmanouil A.; Baltrus, David A.; Bull, Carolee Theresa; Wechter, William Patrick; Yan, Shuangchun; Ververidis, Filippos; Almeida, Nalvo F.; Jones, Corbin D.; Dangl, Jeffery L.; Panopoulos, Nickolas J.; Vinatzer, Boris A.; Goumas, Dimitrios E.

In: PloS one, Vol. 8, No. 3, e59366, 28.03.2013.

Research output: Contribution to journalArticle

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T1 - Comparative Genomics of Multiple Strains of Pseudomonas cannabina pv. alisalensis, a Potential Model Pathogen of Both Monocots and Dicots

AU - Sarris, Panagiotis F.

AU - Trantas, Emmanouil A.

AU - Baltrus, David A.

AU - Bull, Carolee Theresa

AU - Wechter, William Patrick

AU - Yan, Shuangchun

AU - Ververidis, Filippos

AU - Almeida, Nalvo F.

AU - Jones, Corbin D.

AU - Dangl, Jeffery L.

AU - Panopoulos, Nickolas J.

AU - Vinatzer, Boris A.

AU - Goumas, Dimitrios E.

PY - 2013/3/28

Y1 - 2013/3/28

N2 - Comparative genomics of closely related pathogens that differ in host range can provide insights into mechanisms of host-pathogen interactions and host adaptation. Furthermore, sequencing of multiple strains with the same host range reveals information concerning pathogen diversity and the molecular basis of virulence. Here we present a comparative analysis of draft genome sequences for four strains of Pseudomonas cannabina pathovar alisalensis (Pcal), which is pathogenic on a range of monocotyledonous and dicotyledonous plants. These draft genome sequences provide a foundation for understanding host range evolution across the monocot-dicot divide. Like other phytopathogenic pseudomonads, Pcal strains harboured a hrp/hrc gene cluster that codes for a type III secretion system. Phylogenetic analysis based on the hrp/hrc cluster genes/proteins, suggests localized recombination and functional divergence within the hrp/hrc cluster. Despite significant conservation of overall genetic content across Pcal genomes, comparison of type III effector repertoires reinforced previous molecular data suggesting the existence of two distinct lineages within this pathovar. Furthermore, all Pcal strains analyzed harbored two distinct genomic islands predicted to code for type VI secretion systems (T6SSs). While one of these systems was orthologous to known P. syringae T6SSs, the other more closely resembled a T6SS found within P. aeruginosa. In summary, our study provides a foundation to unravel Pcal adaptation to both monocot and dicot hosts and provides genetic insights into the mechanisms underlying pathogenicity.

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