Comparative structural and functional analysis of the olfactory receptor genes flanking the human and mouse β-globin gene clusters

Michael Bulger, M. A. Bender, J. Hikke Van Doorninck, Brett Wertman, Catherine M. Farrell, Gary Felsenfeld, Mark Groudine, Ross Hardison

Research output: Contribution to journalArticle

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Abstract

By sequencing regions flanking the β-globin gene complex in mouse (Hbbc) and human (HBBC), we have shown that the β-globin gene cluster is surrounded by a larger cluster of olfactory receptor genes (ORGs). To facilitate sequence comparisons and to investigate the regulation of ORG expression, we have mapped 5′ sequences of mRNA from olfactory epithelium encoding β-globin-proximal ORGs. We have found that several of these genes contain multiple noncoding exons that can be alternatively spliced. Surprisingly, the only common motifs found in the promoters of these genes are a "TATA" box and a purine-rich motif. Sequence comparisons between human and mouse reveal that most of the conserved regions are confined to the coding regions and transcription units of the genes themselves, but a few blocks of conserved sequence also are found outside of ORG transcription units. The possible influence of β-globin regulatory sequences on ORG expression in olfactory epithelium was tested in mice containing a deletion of the endogenous β-globin locus control region, but no change in expression of the neighboring ORGs was detected. We evaluate the implications of these results for possible mechanisms of regulation of ORG transcription.

Original languageEnglish (US)
Pages (from-to)14560-14565
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number26
DOIs
StatePublished - Dec 19 2000

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Odorant Receptors
Globins
Multigene Family
Genes
Olfactory Mucosa
Locus Control Region
Gene Expression
TATA Box
Conserved Sequence
Exons

All Science Journal Classification (ASJC) codes

  • General

Cite this

Bulger, Michael ; Bender, M. A. ; Van Doorninck, J. Hikke ; Wertman, Brett ; Farrell, Catherine M. ; Felsenfeld, Gary ; Groudine, Mark ; Hardison, Ross. / Comparative structural and functional analysis of the olfactory receptor genes flanking the human and mouse β-globin gene clusters. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 26. pp. 14560-14565.
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Comparative structural and functional analysis of the olfactory receptor genes flanking the human and mouse β-globin gene clusters. / Bulger, Michael; Bender, M. A.; Van Doorninck, J. Hikke; Wertman, Brett; Farrell, Catherine M.; Felsenfeld, Gary; Groudine, Mark; Hardison, Ross.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 26, 19.12.2000, p. 14560-14565.

Research output: Contribution to journalArticle

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