Comparative studies on the distribution of protein-o-carboxylmethyltransferase and tyrosine hydroxylase in rat brain by immunocytochemistry

M. L. Billingsley, C. D. Balaban, U. Berresheim, D. M. Kuhn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The distribution of protein-O-carboxylmethyltransferase and tyrosine hydroxylase immunoreactivity in brain was compared with the use of highly specific polyclonal antibodies prepared against the native form of each enzyme. Protein-O-carboxylmethyltransferase was found in brain areas rich in catecholamine neurons as identified by tyrosine hydroxylase immunoreactivity. Rabbit anti-protein-O-carboxylmethyltransferase labeled cell bodies in the locus coeruleus, substantia nigra, and paraventricular nucleus whereas rabbit anti-tyrosine hydroxylase prepared against highly purified, native tyrosine hydroxylase from cultured PC12 cells labelled cell bodies in the same brain regions. In addition, the antibody to tyrosine hydroxylase made possible the visualization of very fine cortical processes containing tyrosine hydroxylase and very dense neuronal networks throughout the nigrostriatal pathway. The coincidence of protein-O-carboxylmethyltransferase and tyrosine hydroxylase in catecholamine rich brain areas provide an anatomical basis for the possibility that protein-O-carboxylmethyltransferase could modulate catecholaminergic neurotransmission.

Original languageEnglish (US)
Pages (from-to)255-265
Number of pages11
JournalNeurochemistry International
Volume8
Issue number2
DOIs
StatePublished - 1986

Fingerprint

Protein O-Methyltransferase
Tyrosine 3-Monooxygenase
Immunohistochemistry
Brain
Catecholamines
Rabbits
Locus Coeruleus
Antibodies
Paraventricular Hypothalamic Nucleus
PC12 Cells
Substantia Nigra
Synaptic Transmission
Cultured Cells
Neurons

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

@article{25fbca97e7f040b0ab0c958e5d3a42c9,
title = "Comparative studies on the distribution of protein-o-carboxylmethyltransferase and tyrosine hydroxylase in rat brain by immunocytochemistry",
abstract = "The distribution of protein-O-carboxylmethyltransferase and tyrosine hydroxylase immunoreactivity in brain was compared with the use of highly specific polyclonal antibodies prepared against the native form of each enzyme. Protein-O-carboxylmethyltransferase was found in brain areas rich in catecholamine neurons as identified by tyrosine hydroxylase immunoreactivity. Rabbit anti-protein-O-carboxylmethyltransferase labeled cell bodies in the locus coeruleus, substantia nigra, and paraventricular nucleus whereas rabbit anti-tyrosine hydroxylase prepared against highly purified, native tyrosine hydroxylase from cultured PC12 cells labelled cell bodies in the same brain regions. In addition, the antibody to tyrosine hydroxylase made possible the visualization of very fine cortical processes containing tyrosine hydroxylase and very dense neuronal networks throughout the nigrostriatal pathway. The coincidence of protein-O-carboxylmethyltransferase and tyrosine hydroxylase in catecholamine rich brain areas provide an anatomical basis for the possibility that protein-O-carboxylmethyltransferase could modulate catecholaminergic neurotransmission.",
author = "Billingsley, {M. L.} and Balaban, {C. D.} and U. Berresheim and Kuhn, {D. M.}",
year = "1986",
doi = "10.1016/0197-0186(86)90172-5",
language = "English (US)",
volume = "8",
pages = "255--265",
journal = "Neurochemistry International",
issn = "0197-0186",
publisher = "Elsevier Limited",
number = "2",

}

Comparative studies on the distribution of protein-o-carboxylmethyltransferase and tyrosine hydroxylase in rat brain by immunocytochemistry. / Billingsley, M. L.; Balaban, C. D.; Berresheim, U.; Kuhn, D. M.

In: Neurochemistry International, Vol. 8, No. 2, 1986, p. 255-265.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparative studies on the distribution of protein-o-carboxylmethyltransferase and tyrosine hydroxylase in rat brain by immunocytochemistry

AU - Billingsley, M. L.

AU - Balaban, C. D.

AU - Berresheim, U.

AU - Kuhn, D. M.

PY - 1986

Y1 - 1986

N2 - The distribution of protein-O-carboxylmethyltransferase and tyrosine hydroxylase immunoreactivity in brain was compared with the use of highly specific polyclonal antibodies prepared against the native form of each enzyme. Protein-O-carboxylmethyltransferase was found in brain areas rich in catecholamine neurons as identified by tyrosine hydroxylase immunoreactivity. Rabbit anti-protein-O-carboxylmethyltransferase labeled cell bodies in the locus coeruleus, substantia nigra, and paraventricular nucleus whereas rabbit anti-tyrosine hydroxylase prepared against highly purified, native tyrosine hydroxylase from cultured PC12 cells labelled cell bodies in the same brain regions. In addition, the antibody to tyrosine hydroxylase made possible the visualization of very fine cortical processes containing tyrosine hydroxylase and very dense neuronal networks throughout the nigrostriatal pathway. The coincidence of protein-O-carboxylmethyltransferase and tyrosine hydroxylase in catecholamine rich brain areas provide an anatomical basis for the possibility that protein-O-carboxylmethyltransferase could modulate catecholaminergic neurotransmission.

AB - The distribution of protein-O-carboxylmethyltransferase and tyrosine hydroxylase immunoreactivity in brain was compared with the use of highly specific polyclonal antibodies prepared against the native form of each enzyme. Protein-O-carboxylmethyltransferase was found in brain areas rich in catecholamine neurons as identified by tyrosine hydroxylase immunoreactivity. Rabbit anti-protein-O-carboxylmethyltransferase labeled cell bodies in the locus coeruleus, substantia nigra, and paraventricular nucleus whereas rabbit anti-tyrosine hydroxylase prepared against highly purified, native tyrosine hydroxylase from cultured PC12 cells labelled cell bodies in the same brain regions. In addition, the antibody to tyrosine hydroxylase made possible the visualization of very fine cortical processes containing tyrosine hydroxylase and very dense neuronal networks throughout the nigrostriatal pathway. The coincidence of protein-O-carboxylmethyltransferase and tyrosine hydroxylase in catecholamine rich brain areas provide an anatomical basis for the possibility that protein-O-carboxylmethyltransferase could modulate catecholaminergic neurotransmission.

UR - http://www.scopus.com/inward/record.url?scp=0022573090&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022573090&partnerID=8YFLogxK

U2 - 10.1016/0197-0186(86)90172-5

DO - 10.1016/0197-0186(86)90172-5

M3 - Article

AN - SCOPUS:0022573090

VL - 8

SP - 255

EP - 265

JO - Neurochemistry International

JF - Neurochemistry International

SN - 0197-0186

IS - 2

ER -