Comparison of arterial and venous blood-gas values in anesthetized Dorset cross-bred lambs (Ovis aries) using a point-of-care analyzer

Jenelle Izer, Erin Mattern, Joy Ellwanger, Ronald Wilson

Research output: Contribution to journalArticle

Abstract

Objective: To determine the degree of agreement between arterial and venous blood gases in anesthetized lambs using a point-of-care analyzer. Study design: Prospective experimental study. Animals: A group of 12 female Dorset cross-bred lambs, weighing 37.3 ± 7.4 kg (mean ± standard deviation). Methods: Lambs were anesthetized with isoflurane for catheterization of the jugular vein and femoral artery, and blood samples were collected simultaneously for analysis of pH, PCO 2 , PO 2 , base excess (BE), bicarbonate (HCO 3 ), total carbon dioxide (tCO 2 ), oxygen saturation (SO 2 ), ionized calcium (iCa) and potassium (K + ) using the Vet Scan i-STAT handheld analyzer. The Bland–Altman method was used to calculate agreement between arterial and venous measurements. Results: Strong agreement was identified between arterial and venous pH (bias = 0.04; 95% limits of agreement = 0.02–0.06), K + (bias = –0.1 and 95% limits of agreement = –0.8 to 0.5) and iCa (bias = 0.04; 95% limits of agreement = –0.08 to 0.0003). There was poor agreement for PO 2 (bias = 168.0; 95% limits of agreement = 77.4–258.7) and SO 2 (bias = 9.1; 95% limits of agreement = 2.8–15.3), as these values were higher in arterial blood than in venous blood. Moderate agreement was present for BE (bias = 1.7; 95% limits of agreement = –2.7 to 6.1), PCO 2 (bias = –2.1; 95% limits of agreement = –7.5 to 3.4), HCO 3 (bias = 1.1; 95% limits of agreement = –3.1 to 5.3) and tCO 2 (bias = 1.0; 95% limits of agreement = –3.3 to 5.3). Conclusions and clinical relevance: Venous blood can be used for the measurement of pH, K + and iCa in anesthetized Dorset cross-bred lambs. Arterial blood is required for accurate measurement of PO 2 , SO 2 , PCO 2 , HCO 3 , tCO 2 and BE to assess systemic blood oxygenation and pulmonary function in anesthetized Dorset cross-bred lambs.

Original languageEnglish (US)
Pages (from-to)209-213
Number of pages5
JournalVeterinary Anaesthesia and Analgesia
Volume46
Issue number2
DOIs
StatePublished - Mar 1 2019

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Point-of-Care Systems
Domestic Sheep
Dorset (sheep breed)
blood gases
crossbreds
lambs
Gases
sheep
blood
Calcium
calcium
Isoflurane
Jugular Veins
Femoral Artery
Bicarbonates
Carbon Dioxide
Catheterization
lung function
isoflurane
jugular vein

All Science Journal Classification (ASJC) codes

  • veterinary(all)

Cite this

@article{8c44ed39ee2d49e9b79665b9cb6afe74,
title = "Comparison of arterial and venous blood-gas values in anesthetized Dorset cross-bred lambs (Ovis aries) using a point-of-care analyzer",
abstract = "Objective: To determine the degree of agreement between arterial and venous blood gases in anesthetized lambs using a point-of-care analyzer. Study design: Prospective experimental study. Animals: A group of 12 female Dorset cross-bred lambs, weighing 37.3 ± 7.4 kg (mean ± standard deviation). Methods: Lambs were anesthetized with isoflurane for catheterization of the jugular vein and femoral artery, and blood samples were collected simultaneously for analysis of pH, PCO 2 , PO 2 , base excess (BE), bicarbonate (HCO 3 − ), total carbon dioxide (tCO 2 ), oxygen saturation (SO 2 ), ionized calcium (iCa) and potassium (K + ) using the Vet Scan i-STAT handheld analyzer. The Bland–Altman method was used to calculate agreement between arterial and venous measurements. Results: Strong agreement was identified between arterial and venous pH (bias = 0.04; 95{\%} limits of agreement = 0.02–0.06), K + (bias = –0.1 and 95{\%} limits of agreement = –0.8 to 0.5) and iCa (bias = 0.04; 95{\%} limits of agreement = –0.08 to 0.0003). There was poor agreement for PO 2 (bias = 168.0; 95{\%} limits of agreement = 77.4–258.7) and SO 2 (bias = 9.1; 95{\%} limits of agreement = 2.8–15.3), as these values were higher in arterial blood than in venous blood. Moderate agreement was present for BE (bias = 1.7; 95{\%} limits of agreement = –2.7 to 6.1), PCO 2 (bias = –2.1; 95{\%} limits of agreement = –7.5 to 3.4), HCO 3 − (bias = 1.1; 95{\%} limits of agreement = –3.1 to 5.3) and tCO 2 (bias = 1.0; 95{\%} limits of agreement = –3.3 to 5.3). Conclusions and clinical relevance: Venous blood can be used for the measurement of pH, K + and iCa in anesthetized Dorset cross-bred lambs. Arterial blood is required for accurate measurement of PO 2 , SO 2 , PCO 2 , HCO 3 − , tCO 2 and BE to assess systemic blood oxygenation and pulmonary function in anesthetized Dorset cross-bred lambs.",
author = "Jenelle Izer and Erin Mattern and Joy Ellwanger and Ronald Wilson",
year = "2019",
month = "3",
day = "1",
doi = "10.1016/j.vaa.2018.12.003",
language = "English (US)",
volume = "46",
pages = "209--213",
journal = "Veterinary Anaesthesia and Analgesia",
issn = "1467-2987",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Comparison of arterial and venous blood-gas values in anesthetized Dorset cross-bred lambs (Ovis aries) using a point-of-care analyzer

AU - Izer, Jenelle

AU - Mattern, Erin

AU - Ellwanger, Joy

AU - Wilson, Ronald

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Objective: To determine the degree of agreement between arterial and venous blood gases in anesthetized lambs using a point-of-care analyzer. Study design: Prospective experimental study. Animals: A group of 12 female Dorset cross-bred lambs, weighing 37.3 ± 7.4 kg (mean ± standard deviation). Methods: Lambs were anesthetized with isoflurane for catheterization of the jugular vein and femoral artery, and blood samples were collected simultaneously for analysis of pH, PCO 2 , PO 2 , base excess (BE), bicarbonate (HCO 3 − ), total carbon dioxide (tCO 2 ), oxygen saturation (SO 2 ), ionized calcium (iCa) and potassium (K + ) using the Vet Scan i-STAT handheld analyzer. The Bland–Altman method was used to calculate agreement between arterial and venous measurements. Results: Strong agreement was identified between arterial and venous pH (bias = 0.04; 95% limits of agreement = 0.02–0.06), K + (bias = –0.1 and 95% limits of agreement = –0.8 to 0.5) and iCa (bias = 0.04; 95% limits of agreement = –0.08 to 0.0003). There was poor agreement for PO 2 (bias = 168.0; 95% limits of agreement = 77.4–258.7) and SO 2 (bias = 9.1; 95% limits of agreement = 2.8–15.3), as these values were higher in arterial blood than in venous blood. Moderate agreement was present for BE (bias = 1.7; 95% limits of agreement = –2.7 to 6.1), PCO 2 (bias = –2.1; 95% limits of agreement = –7.5 to 3.4), HCO 3 − (bias = 1.1; 95% limits of agreement = –3.1 to 5.3) and tCO 2 (bias = 1.0; 95% limits of agreement = –3.3 to 5.3). Conclusions and clinical relevance: Venous blood can be used for the measurement of pH, K + and iCa in anesthetized Dorset cross-bred lambs. Arterial blood is required for accurate measurement of PO 2 , SO 2 , PCO 2 , HCO 3 − , tCO 2 and BE to assess systemic blood oxygenation and pulmonary function in anesthetized Dorset cross-bred lambs.

AB - Objective: To determine the degree of agreement between arterial and venous blood gases in anesthetized lambs using a point-of-care analyzer. Study design: Prospective experimental study. Animals: A group of 12 female Dorset cross-bred lambs, weighing 37.3 ± 7.4 kg (mean ± standard deviation). Methods: Lambs were anesthetized with isoflurane for catheterization of the jugular vein and femoral artery, and blood samples were collected simultaneously for analysis of pH, PCO 2 , PO 2 , base excess (BE), bicarbonate (HCO 3 − ), total carbon dioxide (tCO 2 ), oxygen saturation (SO 2 ), ionized calcium (iCa) and potassium (K + ) using the Vet Scan i-STAT handheld analyzer. The Bland–Altman method was used to calculate agreement between arterial and venous measurements. Results: Strong agreement was identified between arterial and venous pH (bias = 0.04; 95% limits of agreement = 0.02–0.06), K + (bias = –0.1 and 95% limits of agreement = –0.8 to 0.5) and iCa (bias = 0.04; 95% limits of agreement = –0.08 to 0.0003). There was poor agreement for PO 2 (bias = 168.0; 95% limits of agreement = 77.4–258.7) and SO 2 (bias = 9.1; 95% limits of agreement = 2.8–15.3), as these values were higher in arterial blood than in venous blood. Moderate agreement was present for BE (bias = 1.7; 95% limits of agreement = –2.7 to 6.1), PCO 2 (bias = –2.1; 95% limits of agreement = –7.5 to 3.4), HCO 3 − (bias = 1.1; 95% limits of agreement = –3.1 to 5.3) and tCO 2 (bias = 1.0; 95% limits of agreement = –3.3 to 5.3). Conclusions and clinical relevance: Venous blood can be used for the measurement of pH, K + and iCa in anesthetized Dorset cross-bred lambs. Arterial blood is required for accurate measurement of PO 2 , SO 2 , PCO 2 , HCO 3 − , tCO 2 and BE to assess systemic blood oxygenation and pulmonary function in anesthetized Dorset cross-bred lambs.

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U2 - 10.1016/j.vaa.2018.12.003

DO - 10.1016/j.vaa.2018.12.003

M3 - Article

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VL - 46

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JO - Veterinary Anaesthesia and Analgesia

JF - Veterinary Anaesthesia and Analgesia

SN - 1467-2987

IS - 2

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