Comparison of continuous fermentations in biofilm and suspended-cell bioreactors for production of human lysozyme

Duygu Ercan, Ali Demirci

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Lysozyme is a lytic enzyme, which has antimicrobial activity. It has been used for food and pharmaceutical applications. Lysozyme production needs to be enhanced to meet its current demand in the industry, because currently egg is used for commercial lysozyme production and it poses immunological problems and it is four-times less effective than human lysozyme. In our earlier study, biofilm reactors have been evaluated for human lysozyme production by Kluyveromyces lactis K7 in batch fermentation, which demonstrated significant improvement in production of human lysozyme. Therefore, this study was undertaken to evaluate continuous fermentations for the human lysozyme production by K. lactis K7 in biofilm reactor and compare with continuous fermentation in suspended cell bioreactor. For this purpose, continuous fermentation was conducted at different dilution rates (0.03-0.08 h-1) in both biofilm and suspended cell reactors. As a result, biofilm reactor provided significantly higher productivity (7.5 U/ml/h) with 0.0055 h-1 of dilution rate compared to the maximum productivity in suspended-cell bioreactor (4 U/ml/h) with 0.004 h-1 of dilution rate. In conclusion, biofilm reactors provided operation at higher dilution rates because of the immobilized cells compared to suspended cell reactor.

Original languageEnglish (US)
Title of host publicationAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014
PublisherAmerican Society of Agricultural and Biological Engineers
Pages1310-1317
Number of pages8
Volume2
ISBN (Electronic)9781632668455
StatePublished - Jan 1 2014
EventAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014 - Montreal, Canada
Duration: Jul 13 2014Jul 16 2014

Other

OtherAmerican Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014
CountryCanada
CityMontreal
Period7/13/147/16/14

Fingerprint

continuous fermentation
Biofilms
Bioreactors
Muramidase
bioreactors
lysozyme
biofilm
Fermentation
Enzymes
Dilution
Kluyveromyces marxianus var. lactis
cells
Kluyveromyces
Productivity
Immobilized Cells
batch fermentation
immobilized cells
Reactor operation
Ovum
Industry

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Mechanical Engineering

Cite this

Ercan, D., & Demirci, A. (2014). Comparison of continuous fermentations in biofilm and suspended-cell bioreactors for production of human lysozyme. In American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014 (Vol. 2, pp. 1310-1317). American Society of Agricultural and Biological Engineers.
Ercan, Duygu ; Demirci, Ali. / Comparison of continuous fermentations in biofilm and suspended-cell bioreactors for production of human lysozyme. American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014. Vol. 2 American Society of Agricultural and Biological Engineers, 2014. pp. 1310-1317
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Ercan, D & Demirci, A 2014, Comparison of continuous fermentations in biofilm and suspended-cell bioreactors for production of human lysozyme. in American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014. vol. 2, American Society of Agricultural and Biological Engineers, pp. 1310-1317, American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014, Montreal, Canada, 7/13/14.

Comparison of continuous fermentations in biofilm and suspended-cell bioreactors for production of human lysozyme. / Ercan, Duygu; Demirci, Ali.

American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014. Vol. 2 American Society of Agricultural and Biological Engineers, 2014. p. 1310-1317.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Ercan D, Demirci A. Comparison of continuous fermentations in biofilm and suspended-cell bioreactors for production of human lysozyme. In American Society of Agricultural and Biological Engineers Annual International Meeting 2014, ASABE 2014. Vol. 2. American Society of Agricultural and Biological Engineers. 2014. p. 1310-1317