Comparison of immune responses elicited by recombinant protein and eukaryotic expression plasmid based on histidine rich protein 2 of Plasmodium falciparum

X. Li, Jun Miao, C. F. Xue, R. F. Zhen, Z. X. Liu, X. F. Wang, S. J. Mu

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To identify the immune characteristics of different vaccine prototypes based on HRP2 and to provide experimental evidence for developing P. f. blood stage vaccines. METHODS: BALB/c mice were immunized with recombinant protein TP-HRP2 or eukaryotic expression plasmid pcDNA3.1(-)/HRP2. The kinetics and specificities of antibody responses were analyzed. The proliferation tests of spleen cells were done, and P. f. growth inhibition assays were done with immune sera. RESULTS: The mice immunized with TP-HRP2 in Freund's adjuvant produced high-level and high-specificity antibody response. The antibodies appeared rapidly and lasted for a longer time. Cellular responses were induced simultaneously, and the immune sera could inhibit the development of parasite in IRBCs. The mice immunized with pcDNA3.1(-)/HRP2 produced middle-level antibody response which had some specificity, however, the induction of antibodies required repeated inoculation and a longer duration. Immune cells were well primed and the memorial immune response was obvious but the immune sera had no effect on the growth of P.f. in vitro. CONCLUSION: Both the recombinant protein and plasmid DNA based on HRP2 have different immune characteristics in mice. HRP2 recombinant protein has the potential in practical application.

Original languageEnglish (US)
Pages (from-to)7-10
Number of pages4
JournalZhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Volume19
Issue number1
StatePublished - Jan 1 2001

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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