Comparison of neutralization of BPV‐1 infection of C127 cells and bovine fetal skin xenografts

Shinje Ghim, Neil Christensen, John W. Kreider, A. Bennett Jenson

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

BPV‐I induces focus formation in murine C127 cells and fibropapillomas in bovine fetal skin xenografts. In this study, we compared the specificity of neutralization of BPV‐I in both assay systems, using sera and monoclonal antibodies (MAbs) selected to define neutralizing epitopes. Sera from rabbits and cattle, inoculated with intact BPV‐1 or BPV‐2 virions, neutralize BPV‐1 infectivity in both C127 cells and xenografts. Selected human sera and murine MAbs that react with intact BPV‐1 particles, serum of a rabbit immunized with denatured BPV‐1 particles, and sera from calves vaccinated with a recombinant L1 fusion protein did not neutralize BPV‐1 infection in either system. It was concluded that neutralization of BPV‐1 infection of C127 cells and bovine fetal skin xenografts by hyperimmune sera is specific and concordant for both assay systems, and involves conformational BPV‐1 capsid epitopes.

Original languageEnglish (US)
Pages (from-to)285-289
Number of pages5
JournalInternational Journal of Cancer
Volume49
Issue number2
DOIs
StatePublished - Jan 1 1991

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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