Comparison of sample preparation and analysis using solid-phase extraction and solid-phase microextraction to determine monohydroxy PAH in urine by GC/HRMS

Wenlin Huang, Christopher J. Smith, Charisse J. Walcott, James Grainger, Donald George Jr Patterson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Sample preparation techniques using solid-phase extraction (SPE) and solid-phase microextraction (SPME) are compared for the analysis of monohydroxy polycyclic aromatic hydrocarbons (OHPAH) in human urine. Urine samples spiked with five carbon-13 labeled internal standards are first enzymatically hydrolyzed. Sixteen OHPAH from eight parent compounds (naphthalene, fluorene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[c]phenanthrene, and benz[a]anthracene) are then extracted along with the internal standards by these two different techniques. The analytes are derivatized by a silylating reagent before final analysis. Final separation and detection are performed by temperature-programmed capillary gas chromatography (GC) and high-resolution mass spectrometry (HRMS). The two extraction techniques are compared for sample preparation time, cost, throughput, reinjection possibility, frequency of outliers, matrix interference, signal linearity, and method detection limit. SPE demonstrates major advantages over SPME for most of these aspects.

Original languageEnglish (US)
Pages (from-to)339-351
Number of pages13
JournalPolycyclic Aromatic Compounds
Volume22
Issue number3-4
DOIs
StatePublished - Jan 1 2002

Fingerprint

Polycyclic aromatic hydrocarbons
Gas chromatography
Mass spectrometry
Polycyclic Aromatic Hydrocarbons
Anthracene
Pyrene
Signal interference
Naphthalene
Carbon
Throughput
Costs
Temperature

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

Cite this

Huang, Wenlin ; Smith, Christopher J. ; Walcott, Charisse J. ; Grainger, James ; Patterson, Donald George Jr. / Comparison of sample preparation and analysis using solid-phase extraction and solid-phase microextraction to determine monohydroxy PAH in urine by GC/HRMS. In: Polycyclic Aromatic Compounds. 2002 ; Vol. 22, No. 3-4. pp. 339-351.
@article{fc0f321d15d24f2389c2428cc1aa7d29,
title = "Comparison of sample preparation and analysis using solid-phase extraction and solid-phase microextraction to determine monohydroxy PAH in urine by GC/HRMS",
abstract = "Sample preparation techniques using solid-phase extraction (SPE) and solid-phase microextraction (SPME) are compared for the analysis of monohydroxy polycyclic aromatic hydrocarbons (OHPAH) in human urine. Urine samples spiked with five carbon-13 labeled internal standards are first enzymatically hydrolyzed. Sixteen OHPAH from eight parent compounds (naphthalene, fluorene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[c]phenanthrene, and benz[a]anthracene) are then extracted along with the internal standards by these two different techniques. The analytes are derivatized by a silylating reagent before final analysis. Final separation and detection are performed by temperature-programmed capillary gas chromatography (GC) and high-resolution mass spectrometry (HRMS). The two extraction techniques are compared for sample preparation time, cost, throughput, reinjection possibility, frequency of outliers, matrix interference, signal linearity, and method detection limit. SPE demonstrates major advantages over SPME for most of these aspects.",
author = "Wenlin Huang and Smith, {Christopher J.} and Walcott, {Charisse J.} and James Grainger and Patterson, {Donald George Jr}",
year = "2002",
month = "1",
day = "1",
doi = "10.1080/10406630290027000",
language = "English (US)",
volume = "22",
pages = "339--351",
journal = "Polycyclic Aromatic Compounds",
issn = "1040-6638",
publisher = "Taylor and Francis Ltd.",
number = "3-4",

}

Comparison of sample preparation and analysis using solid-phase extraction and solid-phase microextraction to determine monohydroxy PAH in urine by GC/HRMS. / Huang, Wenlin; Smith, Christopher J.; Walcott, Charisse J.; Grainger, James; Patterson, Donald George Jr.

In: Polycyclic Aromatic Compounds, Vol. 22, No. 3-4, 01.01.2002, p. 339-351.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparison of sample preparation and analysis using solid-phase extraction and solid-phase microextraction to determine monohydroxy PAH in urine by GC/HRMS

AU - Huang, Wenlin

AU - Smith, Christopher J.

AU - Walcott, Charisse J.

AU - Grainger, James

AU - Patterson, Donald George Jr

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Sample preparation techniques using solid-phase extraction (SPE) and solid-phase microextraction (SPME) are compared for the analysis of monohydroxy polycyclic aromatic hydrocarbons (OHPAH) in human urine. Urine samples spiked with five carbon-13 labeled internal standards are first enzymatically hydrolyzed. Sixteen OHPAH from eight parent compounds (naphthalene, fluorene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[c]phenanthrene, and benz[a]anthracene) are then extracted along with the internal standards by these two different techniques. The analytes are derivatized by a silylating reagent before final analysis. Final separation and detection are performed by temperature-programmed capillary gas chromatography (GC) and high-resolution mass spectrometry (HRMS). The two extraction techniques are compared for sample preparation time, cost, throughput, reinjection possibility, frequency of outliers, matrix interference, signal linearity, and method detection limit. SPE demonstrates major advantages over SPME for most of these aspects.

AB - Sample preparation techniques using solid-phase extraction (SPE) and solid-phase microextraction (SPME) are compared for the analysis of monohydroxy polycyclic aromatic hydrocarbons (OHPAH) in human urine. Urine samples spiked with five carbon-13 labeled internal standards are first enzymatically hydrolyzed. Sixteen OHPAH from eight parent compounds (naphthalene, fluorene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[c]phenanthrene, and benz[a]anthracene) are then extracted along with the internal standards by these two different techniques. The analytes are derivatized by a silylating reagent before final analysis. Final separation and detection are performed by temperature-programmed capillary gas chromatography (GC) and high-resolution mass spectrometry (HRMS). The two extraction techniques are compared for sample preparation time, cost, throughput, reinjection possibility, frequency of outliers, matrix interference, signal linearity, and method detection limit. SPE demonstrates major advantages over SPME for most of these aspects.

UR - http://www.scopus.com/inward/record.url?scp=0036025791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036025791&partnerID=8YFLogxK

U2 - 10.1080/10406630290027000

DO - 10.1080/10406630290027000

M3 - Article

AN - SCOPUS:0036025791

VL - 22

SP - 339

EP - 351

JO - Polycyclic Aromatic Compounds

JF - Polycyclic Aromatic Compounds

SN - 1040-6638

IS - 3-4

ER -