Comparison of the complete genome sequences of Bifidobacterium animalis subsp. lactis DSM 10140 and Bl-04

Rodolphe Barrangou, Elizabeth P. Briczinski, Lindsay L. Traeger, Joseph R. Loquasto, Melissa Richards, Philippe Horvath, Anne Claire Coûté-Monvoisin, Gregory Leyer, Snjezana Rendulic, James L. Steele, Jeffery R. Broadbent, Taylor Oberg, Edward G. Dudley, Stephan Schuster, Dennis A. Romero, Robert F. Roberts

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Bifidobacteria are important members of the human gut flora, especially in infants. Comparative genomic analysis of two Bifidobacterium animalis subsp. lactis strains revealed evolution by internal deletion of consecutive spacer-repeat units within a novel clustered regularly interspaced short palindromic repeat locus, which represented the largest differential content between the two genomes. Additionally, 47 single nucleotide polymorphisms were identified, consisting primarily of nonsynonymous mutations, indicating positive selection and/or recent divergence. A particular nonsynonymous mutation in a putative glucose transporter was linked to a negative phenotypic effect on the ability of the variant to catabolize glucose, consistent with a modification in the predicted protein transmembrane topology. Comparative genome sequence analysis of three Bifidobacterium species provided a core genome set of 1,117 orthologs complemented by a pan-genome of 2,445 genes. The genome sequences of the intestinal bacterium B. animalis subsp. lactis provide insights into rapid genome evolution and the genetic basis for adaptation to the human gut environment, notably with regard to catabolism of dietary carbohydrates, resistance to bile and acid, and interaction with the intestinal epithelium. The high degree of genome conservation observed between the two strains in terms of size, organization, and sequence is indicative of a genomically monomorphic subspecies and explains the inability to differentiate the strains by standard techniques such as pulsed-field gel electrophoresis.

Original languageEnglish (US)
Pages (from-to)4144-4151
Number of pages8
JournalJournal of bacteriology
Volume191
Issue number13
DOIs
StatePublished - Jul 1 2009

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Genome
Bifidobacterium
Clustered Regularly Interspaced Short Palindromic Repeats
Dietary Carbohydrates
Mutation
Molecular Evolution
Facilitative Glucose Transport Proteins
Pulsed Field Gel Electrophoresis
Intestinal Mucosa
Bifidobacterium animalis
Bile Acids and Salts
Single Nucleotide Polymorphism
Sequence Analysis
Bacteria
Glucose
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Barrangou, R., Briczinski, E. P., Traeger, L. L., Loquasto, J. R., Richards, M., Horvath, P., ... Roberts, R. F. (2009). Comparison of the complete genome sequences of Bifidobacterium animalis subsp. lactis DSM 10140 and Bl-04. Journal of bacteriology, 191(13), 4144-4151. https://doi.org/10.1128/JB.00155-09
Barrangou, Rodolphe ; Briczinski, Elizabeth P. ; Traeger, Lindsay L. ; Loquasto, Joseph R. ; Richards, Melissa ; Horvath, Philippe ; Coûté-Monvoisin, Anne Claire ; Leyer, Gregory ; Rendulic, Snjezana ; Steele, James L. ; Broadbent, Jeffery R. ; Oberg, Taylor ; Dudley, Edward G. ; Schuster, Stephan ; Romero, Dennis A. ; Roberts, Robert F. / Comparison of the complete genome sequences of Bifidobacterium animalis subsp. lactis DSM 10140 and Bl-04. In: Journal of bacteriology. 2009 ; Vol. 191, No. 13. pp. 4144-4151.
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Barrangou, R, Briczinski, EP, Traeger, LL, Loquasto, JR, Richards, M, Horvath, P, Coûté-Monvoisin, AC, Leyer, G, Rendulic, S, Steele, JL, Broadbent, JR, Oberg, T, Dudley, EG, Schuster, S, Romero, DA & Roberts, RF 2009, 'Comparison of the complete genome sequences of Bifidobacterium animalis subsp. lactis DSM 10140 and Bl-04', Journal of bacteriology, vol. 191, no. 13, pp. 4144-4151. https://doi.org/10.1128/JB.00155-09

Comparison of the complete genome sequences of Bifidobacterium animalis subsp. lactis DSM 10140 and Bl-04. / Barrangou, Rodolphe; Briczinski, Elizabeth P.; Traeger, Lindsay L.; Loquasto, Joseph R.; Richards, Melissa; Horvath, Philippe; Coûté-Monvoisin, Anne Claire; Leyer, Gregory; Rendulic, Snjezana; Steele, James L.; Broadbent, Jeffery R.; Oberg, Taylor; Dudley, Edward G.; Schuster, Stephan; Romero, Dennis A.; Roberts, Robert F.

In: Journal of bacteriology, Vol. 191, No. 13, 01.07.2009, p. 4144-4151.

Research output: Contribution to journalArticle

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AU - Loquasto, Joseph R.

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AU - Steele, James L.

AU - Broadbent, Jeffery R.

AU - Oberg, Taylor

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AU - Roberts, Robert F.

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Barrangou R, Briczinski EP, Traeger LL, Loquasto JR, Richards M, Horvath P et al. Comparison of the complete genome sequences of Bifidobacterium animalis subsp. lactis DSM 10140 and Bl-04. Journal of bacteriology. 2009 Jul 1;191(13):4144-4151. https://doi.org/10.1128/JB.00155-09