Comparison of the substrate-binding pockets of the Rous sarcoma virus and human immunodeficiency virus type 1 proteases

Craig Eugene Cameron, B. Grinde, P. Jacques, J. Jentoft, J. Leis, A. Wlodawer, I. T. Weber

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A steady state kinetic analysis of the avian myeloblastosis virus/Rous sarcoma virus (AMV/RSV) and human immunodeficiency virus Type 1 (HIV-1) retroviral proteases (PRs) was carried out using a series of 40 peptide substrates that are derivatives of the AMV/RSV nucleocapsid-PR cleavage site. These peptides contain single amino acid substitutions in each of the seven positions of the minimum length substrate required by the PR for specific and efficient cleavage. These peptide substrates are distinguished by the individual enzyme subsites of the AMV/RSV and HIV-1 PRs. The molecular basis for similarities and differences of the individual subsites for both proteases is discussed using steady state kinetic data and modeling based on crystal structures.

Original languageEnglish (US)
Pages (from-to)11711-11720
Number of pages10
JournalJournal of Biological Chemistry
Volume268
Issue number16
StatePublished - Jan 1 1993

Fingerprint

Rous sarcoma virus
Avian Myeloblastosis Virus
Viruses
HIV-1
Peptide Hydrolases
Substrates
Peptides
Nucleocapsid
Amino Acid Substitution
Individuality
Kinetics
Enzymes
Substitution reactions
Crystal structure
Derivatives
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cameron, C. E., Grinde, B., Jacques, P., Jentoft, J., Leis, J., Wlodawer, A., & Weber, I. T. (1993). Comparison of the substrate-binding pockets of the Rous sarcoma virus and human immunodeficiency virus type 1 proteases. Journal of Biological Chemistry, 268(16), 11711-11720.
Cameron, Craig Eugene ; Grinde, B. ; Jacques, P. ; Jentoft, J. ; Leis, J. ; Wlodawer, A. ; Weber, I. T. / Comparison of the substrate-binding pockets of the Rous sarcoma virus and human immunodeficiency virus type 1 proteases. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 16. pp. 11711-11720.
@article{675ee020583c40a3a6db75c0ea854b80,
title = "Comparison of the substrate-binding pockets of the Rous sarcoma virus and human immunodeficiency virus type 1 proteases",
abstract = "A steady state kinetic analysis of the avian myeloblastosis virus/Rous sarcoma virus (AMV/RSV) and human immunodeficiency virus Type 1 (HIV-1) retroviral proteases (PRs) was carried out using a series of 40 peptide substrates that are derivatives of the AMV/RSV nucleocapsid-PR cleavage site. These peptides contain single amino acid substitutions in each of the seven positions of the minimum length substrate required by the PR for specific and efficient cleavage. These peptide substrates are distinguished by the individual enzyme subsites of the AMV/RSV and HIV-1 PRs. The molecular basis for similarities and differences of the individual subsites for both proteases is discussed using steady state kinetic data and modeling based on crystal structures.",
author = "Cameron, {Craig Eugene} and B. Grinde and P. Jacques and J. Jentoft and J. Leis and A. Wlodawer and Weber, {I. T.}",
year = "1993",
month = "1",
day = "1",
language = "English (US)",
volume = "268",
pages = "11711--11720",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "16",

}

Cameron, CE, Grinde, B, Jacques, P, Jentoft, J, Leis, J, Wlodawer, A & Weber, IT 1993, 'Comparison of the substrate-binding pockets of the Rous sarcoma virus and human immunodeficiency virus type 1 proteases', Journal of Biological Chemistry, vol. 268, no. 16, pp. 11711-11720.

Comparison of the substrate-binding pockets of the Rous sarcoma virus and human immunodeficiency virus type 1 proteases. / Cameron, Craig Eugene; Grinde, B.; Jacques, P.; Jentoft, J.; Leis, J.; Wlodawer, A.; Weber, I. T.

In: Journal of Biological Chemistry, Vol. 268, No. 16, 01.01.1993, p. 11711-11720.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparison of the substrate-binding pockets of the Rous sarcoma virus and human immunodeficiency virus type 1 proteases

AU - Cameron, Craig Eugene

AU - Grinde, B.

AU - Jacques, P.

AU - Jentoft, J.

AU - Leis, J.

AU - Wlodawer, A.

AU - Weber, I. T.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - A steady state kinetic analysis of the avian myeloblastosis virus/Rous sarcoma virus (AMV/RSV) and human immunodeficiency virus Type 1 (HIV-1) retroviral proteases (PRs) was carried out using a series of 40 peptide substrates that are derivatives of the AMV/RSV nucleocapsid-PR cleavage site. These peptides contain single amino acid substitutions in each of the seven positions of the minimum length substrate required by the PR for specific and efficient cleavage. These peptide substrates are distinguished by the individual enzyme subsites of the AMV/RSV and HIV-1 PRs. The molecular basis for similarities and differences of the individual subsites for both proteases is discussed using steady state kinetic data and modeling based on crystal structures.

AB - A steady state kinetic analysis of the avian myeloblastosis virus/Rous sarcoma virus (AMV/RSV) and human immunodeficiency virus Type 1 (HIV-1) retroviral proteases (PRs) was carried out using a series of 40 peptide substrates that are derivatives of the AMV/RSV nucleocapsid-PR cleavage site. These peptides contain single amino acid substitutions in each of the seven positions of the minimum length substrate required by the PR for specific and efficient cleavage. These peptide substrates are distinguished by the individual enzyme subsites of the AMV/RSV and HIV-1 PRs. The molecular basis for similarities and differences of the individual subsites for both proteases is discussed using steady state kinetic data and modeling based on crystal structures.

UR - http://www.scopus.com/inward/record.url?scp=0027232325&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027232325&partnerID=8YFLogxK

M3 - Article

VL - 268

SP - 11711

EP - 11720

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 16

ER -

Cameron CE, Grinde B, Jacques P, Jentoft J, Leis J, Wlodawer A et al. Comparison of the substrate-binding pockets of the Rous sarcoma virus and human immunodeficiency virus type 1 proteases. Journal of Biological Chemistry. 1993 Jan 1;268(16):11711-11720.