Compartmented neuron cultures for unit 26.4 directional infection by alpha herpesviruses

Dušica Curanović, Toh Hean Ch'ng, Moriah Louise Szpara, Lynn Enquist

Research output: Contribution to journalReview article

20 Citations (Scopus)

Abstract

Compartmented neuronal cultures allow experimenters to establish separate fluid environments for neuronal axons and the soma from which they emanate. Physical isolation of cell bodies and axons is achieved by culturing neurons in tri-chambered Teflon rings. Dissociated ganglia are plated in one end compartment of the trichamber, and axonal growth is guided underneath watertight silicone grease barriers into a separate compartment. Since the axons and cell bodies are located in different compartments, they can be infected and assayed separately. We describe the assembly and use of compartmented neuronal cultures for in vitro study of directional infection of neurons by alpha herpesviruses. Selective application of viral inoculum to only one compartment ensures that the remainder of the neuron is not contaminated by input inoculum. This allows for quantification of viral spread, and unambiguous interpretation of immunofluorescence and electron microscopy images.

Original languageEnglish (US)
JournalCurrent Protocols in Cell Biology
Issue numberSUPPL. 43
DOIs
StatePublished - Jun 24 2009

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Herpesviridae Infections
Axons
Neurons
Herpesviridae
Polytetrafluoroethylene
Carisoprodol
Silicones
Fluorescence Microscopy
Ganglia
Electron Microscopy
Growth
Infection
Cell Body

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

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Compartmented neuron cultures for unit 26.4 directional infection by alpha herpesviruses. / Curanović, Dušica; Ch'ng, Toh Hean; Szpara, Moriah Louise; Enquist, Lynn.

In: Current Protocols in Cell Biology, No. SUPPL. 43, 24.06.2009.

Research output: Contribution to journalReview article

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