@article{c93e896b47e047d3be7b65735dcc6d87,
title = "Compensatory sequence variation between trans-species small rnas and their target sites",
abstract = "Trans-species small regulatory RNAs (sRNAs) are delivered to host plants from diverse pathogens and parasites and can target host mRNAs. How trans-species sRNAs can be effective on diverse hosts has been unclear. Multiple species of the parasitic plant Cuscuta produce trans-species sRNAs that collectively target many host mRNAs. Confirmed target sites are nearly always in highly conserved, protein-coding regions of host mRNAs. Cuscuta trans-species sRNAs can be grouped into superfamilies that have variation in a three-nucleotide period. These variants compensate for synonymous-site variation in host mRNAs. By targeting host mRNAs at highly conserved protein-coding sites, and simultaneously expressing multiple variants to cover synonymous-site variation, Cuscuta trans-species sRNAs may be able to successfully target multiple homologous mRNAs from diverse hosts.",
author = "Johnson, {Nathan R.} and {de Pamphilis}, {Claude W.} and Axtell, {Michael J.}",
note = "Funding Information: We thank T Phifer for help generating preliminary data; LS Berghard for greenhouse support; C Praul for next-generation sequencing support; J Westwood (Virginia Tech) for providing multiple Cuscuta seed stocks and thoughtful insight into the work; C Depew for informing us about C. grono-vii locations in the wild; and M Schon and M Nodine for early access to the NanoPARE method. This work was supported by an award from the United States Department of Agriculture - National Institute of Food and Agriculture [grant number 2018-67013-285] and a Graduate Research Initiative grant (GRI) from the Huck Institutes of the Life Sciences at Penn State. Funding Information: We thank T Phifer for help generating preliminary data; LS Berghard for greenhouse support; C Praul for next-generation sequencing support; J Westwood (Virginia Tech) for providing multiple Cuscuta seed stocks and thoughtful insight into the work; C Depew for informing us about C. gronovii locations in the wild; and M Schon and M Nodine for early access to the NanoPARE method. This work was supported by an award from the United States Department of Agriculture-National Institute of Food and Agriculture [grant number 2018-67013-285] and a Graduate Research Initiative grant (GRI) from the Huck Institutes of the Life Sciences at Penn State. National Institute of Food and Agriculture 2018-67013-28514 Michael J Axtell Claude W dePamphilis. Publisher Copyright: {\textcopyright} 2019, eLife Sciences Publications Ltd. All rights reserved.",
year = "2019",
month = dec,
doi = "10.7554/eLife.49750",
language = "English (US)",
volume = "8",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}