Complete and incomplete hepatitis B virus particles: Formation, function, and application

Jianming Hu, Kuancheng Liu

Research output: Contribution to journalReview article

59 Citations (Scopus)

Abstract

Hepatitis B virus (HBV) is a para-retrovirus or retroid virus that contains a double-stranded DNA genome and replicates this DNA via reverse transcription of a RNA pregenome. Viral reverse transcription takes place within a capsid upon packaging of the RNA and the viral reverse transcriptase. A major characteristic of HBV replication is the selection of capsids containing the double-stranded DNA, but not those containing the RNA or the single-stranded DNA replication intermediate, for envelopment during virion secretion. The complete HBV virion particles thus contain an outer envelope, studded with viral envelope proteins, that encloses the capsid, which, in turn, encapsidates the double-stranded DNA genome. Furthermore, HBV morphogenesis is characterized by the release of subviral particles that are several orders of magnitude more abundant than the complete virions. One class of subviral particles are the classical surface antigen particles (Australian antigen) that contain only the viral envelope proteins, whereas the more recently discovered genome-free (empty) virions contain both the envelope and capsid but no genome. In addition, recent evidence suggests that low levels of RNA-containing particles may be released, after all. We will summarize what is currently known about how the complete and incomplete HBV particles are assembled. We will discuss briefly the functions of the subviral particles, which remain largely unknown. Finally, we will explore the utility of the subviral particles, particularly, the potential of empty virions and putative RNA virions as diagnostic markers and the potential of empty virons as a vaccine candidate.

Original languageEnglish (US)
Article number56
JournalViruses
Volume9
Issue number3
DOIs
StatePublished - Mar 21 2017

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Defective Viruses
Hepatitis B virus
Virion
Capsid
Genome
RNA
Viral Envelope Proteins
DNA
Reverse Transcription
Virus Assembly
Single-Stranded DNA
RNA-Directed DNA Polymerase
Viral RNA
Retroviridae
Surface Antigens
Virus Replication
DNA Replication
Morphogenesis
Vaccines
Viruses

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

Cite this

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title = "Complete and incomplete hepatitis B virus particles: Formation, function, and application",
abstract = "Hepatitis B virus (HBV) is a para-retrovirus or retroid virus that contains a double-stranded DNA genome and replicates this DNA via reverse transcription of a RNA pregenome. Viral reverse transcription takes place within a capsid upon packaging of the RNA and the viral reverse transcriptase. A major characteristic of HBV replication is the selection of capsids containing the double-stranded DNA, but not those containing the RNA or the single-stranded DNA replication intermediate, for envelopment during virion secretion. The complete HBV virion particles thus contain an outer envelope, studded with viral envelope proteins, that encloses the capsid, which, in turn, encapsidates the double-stranded DNA genome. Furthermore, HBV morphogenesis is characterized by the release of subviral particles that are several orders of magnitude more abundant than the complete virions. One class of subviral particles are the classical surface antigen particles (Australian antigen) that contain only the viral envelope proteins, whereas the more recently discovered genome-free (empty) virions contain both the envelope and capsid but no genome. In addition, recent evidence suggests that low levels of RNA-containing particles may be released, after all. We will summarize what is currently known about how the complete and incomplete HBV particles are assembled. We will discuss briefly the functions of the subviral particles, which remain largely unknown. Finally, we will explore the utility of the subviral particles, particularly, the potential of empty virions and putative RNA virions as diagnostic markers and the potential of empty virons as a vaccine candidate.",
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Complete and incomplete hepatitis B virus particles : Formation, function, and application. / Hu, Jianming; Liu, Kuancheng.

In: Viruses, Vol. 9, No. 3, 56, 21.03.2017.

Research output: Contribution to journalReview article

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