Complete enzyme set for chlorophyll biosynthesis in Escherichia coli

Guangyu E. Chen, Daniel P. Canniffe, Samuel F.H. Barnett, Sarah Hollingshead, Amanda A. Brindley, Cvetelin Vasilev, Donald A. Bryant, C. Neil Hunter

Research output: Contribution to journalArticle

8 Scopus citations


Chlorophylls are essential cofactors for photosynthesis, which sustains global food chains and oxygen production. Billions of tons of chlorophylls are synthesized annually, yet full understanding of chlorophyll biosynthesis has been hindered by the lack of characterization of the Mg–protoporphyrin IX monomethyl ester oxidative cyclase step, which confers the distinctive green color of these pigments. We demonstrate cyclase activity using heterologously expressed enzyme. Next, we assemble a genetic module that encodes the complete chlorophyll biosynthetic pathway and show that it functions in Escherichia coli. Expression of 12 genes converts endogenous protoporphyrin IX into chlorophyll a, turning E. coli cells green. Our results delineate a minimum set of enzymes required to make chlorophyll and establish a platform for engineering photosynthesis in a heterotrophic model organism.

Original languageEnglish (US)
Article numbereaaq1407
JournalScience Advances
Issue number1
StatePublished - Jan 2018


All Science Journal Classification (ASJC) codes

  • General

Cite this

Chen, G. E., Canniffe, D. P., Barnett, S. F. H., Hollingshead, S., Brindley, A. A., Vasilev, C., Bryant, D. A., & Neil Hunter, C. (2018). Complete enzyme set for chlorophyll biosynthesis in Escherichia coli. Science Advances, 4(1), [eaaq1407].