Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042

Roy R. Chaudhuri, Mohammed Sebaihia, Jon L. Hobman, Mark A. Webber, Denisse L. Leyton, Martin D. Goldberg, Adam F. Cunningham, Anthony Scott-Tucker, Paul R. Ferguson, Christopher M. Thomas, Gad Frankel, Christoph M. Tang, Edward G. Dudley, Ian S. Roberts, David A. Rasko, Mark J. Pallen, Julian Parkhill, James P. Nataro, Nicholas R. Thomson, Ian R. Henderson

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Background:Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation. Methods: In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog™ Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12. Conclusion:This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies.

Original languageEnglish (US)
Article numbere8801
JournalPloS one
Volume5
Issue number1
DOIs
StatePublished - Jan 20 2010

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metabolomics
Escherichia coli
Genes
Genome
genome
Escherichia coli K12
diarrhea
Diarrhea
genomics
life events
Campylobacter
malnutrition
growth retardation
volunteers
Virulence Factors
Microarrays
virulence
polysaccharides
Malnutrition
Polysaccharides

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Chaudhuri, R. R., Sebaihia, M., Hobman, J. L., Webber, M. A., Leyton, D. L., Goldberg, M. D., ... Henderson, I. R. (2010). Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. PloS one, 5(1), [e8801]. https://doi.org/10.1371/journal.pone.0008801
Chaudhuri, Roy R. ; Sebaihia, Mohammed ; Hobman, Jon L. ; Webber, Mark A. ; Leyton, Denisse L. ; Goldberg, Martin D. ; Cunningham, Adam F. ; Scott-Tucker, Anthony ; Ferguson, Paul R. ; Thomas, Christopher M. ; Frankel, Gad ; Tang, Christoph M. ; Dudley, Edward G. ; Roberts, Ian S. ; Rasko, David A. ; Pallen, Mark J. ; Parkhill, Julian ; Nataro, James P. ; Thomson, Nicholas R. ; Henderson, Ian R. / Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. In: PloS one. 2010 ; Vol. 5, No. 1.
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Chaudhuri, RR, Sebaihia, M, Hobman, JL, Webber, MA, Leyton, DL, Goldberg, MD, Cunningham, AF, Scott-Tucker, A, Ferguson, PR, Thomas, CM, Frankel, G, Tang, CM, Dudley, EG, Roberts, IS, Rasko, DA, Pallen, MJ, Parkhill, J, Nataro, JP, Thomson, NR & Henderson, IR 2010, 'Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042', PloS one, vol. 5, no. 1, e8801. https://doi.org/10.1371/journal.pone.0008801

Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. / Chaudhuri, Roy R.; Sebaihia, Mohammed; Hobman, Jon L.; Webber, Mark A.; Leyton, Denisse L.; Goldberg, Martin D.; Cunningham, Adam F.; Scott-Tucker, Anthony; Ferguson, Paul R.; Thomas, Christopher M.; Frankel, Gad; Tang, Christoph M.; Dudley, Edward G.; Roberts, Ian S.; Rasko, David A.; Pallen, Mark J.; Parkhill, Julian; Nataro, James P.; Thomson, Nicholas R.; Henderson, Ian R.

In: PloS one, Vol. 5, No. 1, e8801, 20.01.2010.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042

AU - Chaudhuri, Roy R.

AU - Sebaihia, Mohammed

AU - Hobman, Jon L.

AU - Webber, Mark A.

AU - Leyton, Denisse L.

AU - Goldberg, Martin D.

AU - Cunningham, Adam F.

AU - Scott-Tucker, Anthony

AU - Ferguson, Paul R.

AU - Thomas, Christopher M.

AU - Frankel, Gad

AU - Tang, Christoph M.

AU - Dudley, Edward G.

AU - Roberts, Ian S.

AU - Rasko, David A.

AU - Pallen, Mark J.

AU - Parkhill, Julian

AU - Nataro, James P.

AU - Thomson, Nicholas R.

AU - Henderson, Ian R.

PY - 2010/1/20

Y1 - 2010/1/20

N2 - Background:Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation. Methods: In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog™ Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12. Conclusion:This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies.

AB - Background:Escherichia coli can experience a multifaceted life, in some cases acting as a commensal while in other cases causing intestinal and/or extraintestinal disease. Several studies suggest enteroaggregative E. coli are the predominant cause of E. coli-mediated diarrhea in the developed world and are second only to Campylobacter sp. as a cause of bacterial-mediated diarrhea. Furthermore, enteroaggregative E. coli are a predominant cause of persistent diarrhea in the developing world where infection has been associated with malnourishment and growth retardation. Methods: In this study we determined the complete genomic sequence of E. coli 042, the prototypical member of the enteroaggregative E. coli, which has been shown to cause disease in volunteer studies. We performed genomic and phylogenetic comparisons with other E. coli strains revealing previously uncharacterised virulence factors including a variety of secreted proteins and a capsular polysaccharide biosynthetic locus. In addition, by using Biolog™ Phenotype Microarrays we have provided a full metabolic profiling of E. coli 042 and the non-pathogenic lab strain E. coli K-12. We have highlighted the genetic basis for many of the metabolic differences between E. coli 042 and E. coli K-12. Conclusion:This study provides a genetic context for the vast amount of experimental and epidemiological data published thus far and provides a template for future diagnostic and intervention strategies.

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