Complete genomic sequence of Bacteriophage Felix O1

Jean M. Whichard, Lee A. Weigt, Douglas J. Borris, Ling Ling Li, Qing Zhang, Vivek Kapur, F. William Pierson, Erika J. Lingohr, Yi Min She, Andrew M. Kropinski, Nammalwar Sriranganathan

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.

Original languageEnglish (US)
Pages (from-to)710-730
Number of pages21
JournalViruses
Volume2
Issue number3
DOIs
StatePublished - Mar 1 2010

Fingerprint

Bacteriophages
Transfer RNA
Myoviridae
Erwinia amylovora
Coliphages
Bacterial Toxins
Proteins
Base Composition
Capsid Proteins
DNA-Directed RNA Polymerases
Codon
Salmonella
Base Pairing
Proteomics
Introns
Open Reading Frames
Genome
Infection
Genes

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

Cite this

Whichard, J. M., Weigt, L. A., Borris, D. J., Li, L. L., Zhang, Q., Kapur, V., ... Sriranganathan, N. (2010). Complete genomic sequence of Bacteriophage Felix O1. Viruses, 2(3), 710-730. https://doi.org/10.3390/v2030710
Whichard, Jean M. ; Weigt, Lee A. ; Borris, Douglas J. ; Li, Ling Ling ; Zhang, Qing ; Kapur, Vivek ; William Pierson, F. ; Lingohr, Erika J. ; She, Yi Min ; Kropinski, Andrew M. ; Sriranganathan, Nammalwar. / Complete genomic sequence of Bacteriophage Felix O1. In: Viruses. 2010 ; Vol. 2, No. 3. pp. 710-730.
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Whichard, JM, Weigt, LA, Borris, DJ, Li, LL, Zhang, Q, Kapur, V, William Pierson, F, Lingohr, EJ, She, YM, Kropinski, AM & Sriranganathan, N 2010, 'Complete genomic sequence of Bacteriophage Felix O1', Viruses, vol. 2, no. 3, pp. 710-730. https://doi.org/10.3390/v2030710

Complete genomic sequence of Bacteriophage Felix O1. / Whichard, Jean M.; Weigt, Lee A.; Borris, Douglas J.; Li, Ling Ling; Zhang, Qing; Kapur, Vivek; William Pierson, F.; Lingohr, Erika J.; She, Yi Min; Kropinski, Andrew M.; Sriranganathan, Nammalwar.

In: Viruses, Vol. 2, No. 3, 01.03.2010, p. 710-730.

Research output: Contribution to journalArticle

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Whichard JM, Weigt LA, Borris DJ, Li LL, Zhang Q, Kapur V et al. Complete genomic sequence of Bacteriophage Felix O1. Viruses. 2010 Mar 1;2(3):710-730. https://doi.org/10.3390/v2030710