Complete genomic sequence of Bacteriophage Felix O1

Jean M. Whichard; Lee A. Weigt; Douglas J. Borris; Ling Ling Li; Qing Zhang; Vivek Kapur; F. William Pierson; Erika J. Lingohr; Yi Min She; Andrew M. Kropinski; Nammalwar Sriranganathan

doi:10.3390/v2030710

Complete genomic sequence of Bacteriophage Felix O1

Jean M. Whichard, Lee A. Weigt, Douglas J. Borris, Ling Ling Li, Qing Zhang, Vivek Kapur, F. William Pierson, Erika J. Lingohr, Yi Min She, Andrew M. Kropinski, Nammalwar Sriranganathan

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.

Original language	English (US)
Pages (from-to)	710-730
Number of pages	21
Journal	Viruses
Volume	2
Issue number	3
DOIs	https://doi.org/10.3390/v2030710
State	Published - Mar 2010

All Science Journal Classification (ASJC) codes

Infectious Diseases
Virology

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10.3390/v2030710

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title = "Complete genomic sequence of Bacteriophage Felix O1",

abstract = "Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.",

author = "Whichard, {Jean M.} and Weigt, {Lee A.} and Borris, {Douglas J.} and Li, {Ling Ling} and Qing Zhang and Vivek Kapur and {William Pierson}, F. and Lingohr, {Erika J.} and She, {Yi Min} and Kropinski, {Andrew M.} and Nammalwar Sriranganathan",

year = "2010",

month = mar,

doi = "10.3390/v2030710",

language = "English (US)",

volume = "2",

pages = "710--730",

journal = "Viruses",

issn = "1999-4915",

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AU - Whichard, Jean M.

AU - Weigt, Lee A.

AU - Borris, Douglas J.

AU - Li, Ling Ling

AU - Zhang, Qing

AU - Kapur, Vivek

AU - William Pierson, F.

AU - Lingohr, Erika J.

AU - She, Yi Min

AU - Kropinski, Andrew M.

AU - Sriranganathan, Nammalwar

PY - 2010/3

Y1 - 2010/3

N2 - Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.

AB - Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G+C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage φEa21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.

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Complete genomic sequence of Bacteriophage Felix O1

Abstract

All Science Journal Classification (ASJC) codes

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