Complete genomic sequence of Pasteurella multocida, Pm70

Barbara J. May, Qing Zhang, Lingling Li, Michael L. Paustian, Thomas S. Whittam, Vivek Kapur

Research output: Contribution to journalArticle

266 Citations (Scopus)

Abstract

We present here the complete genome sequence of a common avian clone of Pasteurella multocida, Pm70. The genome of Pm70 is a single circular chromosome 2,257,487 base pairs in length and contains 2,014 predicted coding regions, 6 ribosomal RNA operons, and 57 tRNAs. Genome-scale evolutionary analyses based on pairwise comparisons of 1,197 orthologous sequences between P. multocida, Haemophilus influenzae, and Escherichia coil suggest that P. multocida and H. influenzae diverged ≈270 million years ago and the y subdivision of the proteobacteria radiated about 680 million years ago. Two previously undescribed open reading frames, accounting for ≈1% of the genome, encode large proteins with homology to the virulence-associated filamentous hemagglutinin of Bordetella pertussis. Consistent with the critical role of iron in the survival of many microbial pathogens, in silico and wholegenome microarray analyses identified more than 50 Pm70 genes with a potential role in iron acquisition and metabolism. Overall, the complete genomic sequence and preliminary functional analyses provide a foundation for future research into the mechanisms of pathogenesis and host specificity of this important multispecies pathogen.

Original languageEnglish (US)
Pages (from-to)3460-3465
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number6
DOIs
StatePublished - Mar 13 2001

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Pasteurella multocida
Genome
Haemophilus influenzae
Iron
Escherichia
Proteobacteria
Ribosomal RNA
Chromosomes, Human, Pair 2
Host Specificity
Microarray Analysis
Operon
Transfer RNA
Base Pairing
Computer Simulation
Open Reading Frames
Virulence
Clone Cells
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

May, Barbara J. ; Zhang, Qing ; Li, Lingling ; Paustian, Michael L. ; Whittam, Thomas S. ; Kapur, Vivek. / Complete genomic sequence of Pasteurella multocida, Pm70. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 6. pp. 3460-3465.
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Complete genomic sequence of Pasteurella multocida, Pm70. / May, Barbara J.; Zhang, Qing; Li, Lingling; Paustian, Michael L.; Whittam, Thomas S.; Kapur, Vivek.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 6, 13.03.2001, p. 3460-3465.

Research output: Contribution to journalArticle

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