Complete reversal of acid-induced acute lung injury by blocking of platelet-neutrophil aggregation

Alexander Zarbock, Kai Singbartl, Klaus Ley

Research output: Contribution to journalArticlepeer-review

423 Scopus citations

Abstract

Acute lung injury (ALI) causes high mortality, but its molecular mechanisms are poorly understood. Acid aspiration is a frequent cause of ALI, leading to neutrophil sequestration, increased permeability, and deterioration of gas exchange. We investigated the role of platelet-neutrophil interactions in a murine model of acid-induced ALI. Acid aspiration induced P-selectin-dependent platelet-neutrophil interactions in blood and in lung capillaries. Reducing circulating platelets or blocking P-selectin halted the development of ALI. Bone marrow chimeras showed that platelet, not endothelial, P-selectin was responsible for the injury. The interaction of platelets with neutrophils and endothelia was associated with TXA2 formation, with detrimental effects on permeability and tissue function. Activated platelets induced endothelial expression of ICAM-1 and increased neutrophil adhesion. Inhibition of platelet-neutrophil aggregation improved gas exchange, reduced neutrophil recruitment and permeability, and prolonged survival. The key findings were confirmed in a sepsis-induced model of ALI. These findings may translate into improved clinical treatments for ALI.

Original languageEnglish (US)
Pages (from-to)3211-3219
Number of pages9
JournalJournal of Clinical Investigation
Volume116
Issue number12
DOIs
StatePublished - Dec 1 2006

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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