Complex structure of a maize Myb gene promoter: Functional analysis in transgenic plants

Lyudmila V. Sidorenko, Xianggan Li, Suzy M. Cocciolone, Surinder Chopra, Laura Tagliani, Ben Bowen, Michael Daniels, Thomas Peterson

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The maize P gene encodes a Myb-like transcription factor that regulates synthesis of red flavonoid pigments in floral organs. To study the transcriptional regulation of the P gene, candidate regulatory sequences of the P1-rr gene promoter were identified by Ac insertional mutagenesis and subjected to functional testing in transgenic maize plants. The results indicate that a 561 bp fragment (Pb) encompassing the transcription start site (-235 to +326) supports weak expression of a GUS reporter gene in floral organs, including husk, silk, kernel pericarp, cob and male inflorescence. Two other fragments, located approximately 1 and 5 kb 5' of the transcription start site, increased the levels of GUS activity in floral tissues and thus appear to contain enhancer elements. All of the tested constructs gave similar patterns of GUS expression, suggesting that the 561 bp Pb fragment that is common among the transgene constructs contains regulatory elements that promote activation in floral organs. The basal promoter and proximal enhancer fragments contain putative binding sites for bZip regulatory factors, and a complex arrangement of palindromes including a large inverted repeat of two tRNA-like genes. Possibly, interconversions between linear and cruciform conformations of the palindromes may affect protein/DNA interactions and thereby modulate P1-rr expression.

Original languageEnglish (US)
Pages (from-to)471-482
Number of pages12
JournalPlant Journal
Volume22
Issue number6
DOIs
StatePublished - Jun 1 2000

Fingerprint

myb Genes
Genetically Modified Plants
Zea mays
transgenic plants
Transcription Initiation Site
promoter regions
corn
Genes
Inflorescence
Silk
genes
transcription (genetics)
Insertional Mutagenesis
Regulator Genes
Transfer RNA
insertional mutagenesis
Transgenes
Reporter Genes
enhancer elements
Flavonoids

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Sidorenko, L. V., Li, X., Cocciolone, S. M., Chopra, S., Tagliani, L., Bowen, B., ... Peterson, T. (2000). Complex structure of a maize Myb gene promoter: Functional analysis in transgenic plants. Plant Journal, 22(6), 471-482. https://doi.org/10.1046/j.1365-313X.2000.00750.x
Sidorenko, Lyudmila V. ; Li, Xianggan ; Cocciolone, Suzy M. ; Chopra, Surinder ; Tagliani, Laura ; Bowen, Ben ; Daniels, Michael ; Peterson, Thomas. / Complex structure of a maize Myb gene promoter : Functional analysis in transgenic plants. In: Plant Journal. 2000 ; Vol. 22, No. 6. pp. 471-482.
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Sidorenko, LV, Li, X, Cocciolone, SM, Chopra, S, Tagliani, L, Bowen, B, Daniels, M & Peterson, T 2000, 'Complex structure of a maize Myb gene promoter: Functional analysis in transgenic plants', Plant Journal, vol. 22, no. 6, pp. 471-482. https://doi.org/10.1046/j.1365-313X.2000.00750.x

Complex structure of a maize Myb gene promoter : Functional analysis in transgenic plants. / Sidorenko, Lyudmila V.; Li, Xianggan; Cocciolone, Suzy M.; Chopra, Surinder; Tagliani, Laura; Bowen, Ben; Daniels, Michael; Peterson, Thomas.

In: Plant Journal, Vol. 22, No. 6, 01.06.2000, p. 471-482.

Research output: Contribution to journalArticle

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T1 - Complex structure of a maize Myb gene promoter

T2 - Functional analysis in transgenic plants

AU - Sidorenko, Lyudmila V.

AU - Li, Xianggan

AU - Cocciolone, Suzy M.

AU - Chopra, Surinder

AU - Tagliani, Laura

AU - Bowen, Ben

AU - Daniels, Michael

AU - Peterson, Thomas

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