Composite scaffolds

Bridging nanofiber and microsphere architectures to improve bioactivity of mechanically competent constructs

Justin Lee Brown, M. Sean Peach, Lakshmi S. Nair, Sangamesh G. Kumbar, Cato T. Laurencin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Tissue engineering often benefits from the use of composites to produce an ideal scaffold. We present the focused development of a novel structure that combines the biomimetic properties of nanofibers with the robust mechanical aspects of the sintered microsphere scaffold to produce a composite scaffold that demonstrates an ability to mimic the mechanical environment of trabecular bone while also promoting the phenotype progression of osteoblast progenitor cells. These composite nanofiber/microsphere scaffolds exhibited a mechanical modulus and compressive strength similar to trabecular bone and exhibited degradation resulting in a mass loss of 30% after 24 weeks. The nanofiber portion of these scaffolds was sufficiently porous to allow cell migration throughout the fibrous portion of the scaffold and promoted phenotype progression through focal adhesion kinase-mediated activation of the transcription factor Runx2, control scaffolds not containing nanofibers did not demonstrate extensive cell migration or phenotype progression. Ultimately, the focal adhesion kinase activity on the composite nanofiber/microsphere scaffolds demonstrated causality over the production of the mature osteoblast marker, osteocalcin, and the development of a calcified matrix.

Original languageEnglish (US)
Pages (from-to)1150-1158
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume95
Issue number4
DOIs
StatePublished - Dec 15 2010

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Nanofibers
Bioactivity
Microspheres
Scaffolds
Composite materials
Osteoblasts
Focal Adhesion Protein-Tyrosine Kinases
Bone
Adhesion
Transcription factors
Biomimetics
Scaffolds (biology)
Tissue engineering
Osteocalcin
Compressive strength
Chemical activation
Transcription Factors
Degradation

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

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Composite scaffolds : Bridging nanofiber and microsphere architectures to improve bioactivity of mechanically competent constructs. / Brown, Justin Lee; Peach, M. Sean; Nair, Lakshmi S.; Kumbar, Sangamesh G.; Laurencin, Cato T.

In: Journal of Biomedical Materials Research - Part A, Vol. 95, No. 4, 15.12.2010, p. 1150-1158.

Research output: Contribution to journalArticle

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