Comprehensive Two-Dimensional Gas Chromatography for the Fast Separation and Determination of Pesticides Extracted from Human Serum

Zalyou Liu, Sarath R. Sirimanne, Donald G. Patterson, Larry L. Needham, John B. Phillips

Research output: Contribution to journalArticle

108 Scopus citations

Abstract

A comprehensive two-dimensional gas chromatograph with flame ionization detection was constructed and evaluated for the fast separation and analysis of pesticides. A two-stage thermal desorption modulator served as an interface between the two capillary GC columns. By controlling the temperature of the modulator chamber, all sample substances covering a wide polarity and volatility range were modulated without sample breakthrough. Orthogonal separations were achieved with a nonpolar first column and a moderately polar second column. The system allows fast separations of complex mixtures. When we used the method to analyze pesticides extracted from human samples, we achieved complete separation of 15 pesticides in less than 4 min. The flame ionization detector gave detection limits for particular pesticides that ranged from 1.8 to 3.8 pg on-column. The relative standard deviations were from 6.2% to 8.8% over the linear dynamic range.

Original languageEnglish (US)
Pages (from-to)3086-3092
Number of pages7
JournalAnalytical chemistry
Volume66
Issue number19
DOIs
StatePublished - Oct 1 1994

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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