Conditions for copackaging Rous sarcoma virus and murine leukemia virus Gag proteins during retroviral budding

Robert P. Bennett, John Wills

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Rous sarcoma virus (RSV) and murine leukemia virus (MLV) are examples of distantly related retroviruses that normally do not encounter one another in nature. Their Gag proteins direct particle assembly at the plasma membrane but possess very little sequence similarity. As expected, coexpression of these two Gag proteins did not result in particles that contain both. However, when the N-terminal membrane-binding domain of each molecule was replaced with that of the Src oncoprotein, which is also targeted to the cytoplasmic face of the plasma membrane, efficient copackaging was observed in genetic complementation and coimmunoprecipitation assays. We hypothesize that the RSV and MLV Gag proteins normally use distinct locations on the plasma membrane for particle assembly but otherwise have assembly domains that are sufficiently similar in function (but not sequence) to allow heterologous interactions when these proteins are redirected to a common membrane location.

Original languageEnglish (US)
Pages (from-to)2045-2051
Number of pages7
JournalJournal of virology
Volume73
Issue number3
StatePublished - Mar 4 1999

Fingerprint

Murine leukemia virus
Rous sarcoma virus
gag Gene Products
Murine Leukemia Viruses
Cell Membrane
plasma membrane
Retroviridae
Membranes
proteins
Oncogene Proteins
genetic complementation
Proteins
assays

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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Conditions for copackaging Rous sarcoma virus and murine leukemia virus Gag proteins during retroviral budding. / Bennett, Robert P.; Wills, John.

In: Journal of virology, Vol. 73, No. 3, 04.03.1999, p. 2045-2051.

Research output: Contribution to journalArticle

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