Conserved promoter motif is required for cell cycle timing of dnaX transcription in Caulobacter

Research output: Contribution to journalArticle

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Abstract

Cells use highly regulated transcriptional networks to control temporally regulated events. In the bacterium Caulobacter crescentus, many cellular processes are temporally regulated with respect to the cell cycle, and the genes required for these processes are expressed immediately before the products are needed. Genes encoding factors required for DNA replication, including dnaX, dnaA, dnaN, gyrB, and dnaK, are induced at the G1/S-phase transition. By analyzing mutations in the dnax promoter, we identified a motif between the -10 and -35 regions that is required for proper timing of gene expression. This motif, named RRF (for repression of replication factors), is conserved in the promoters of other coordinately induced replication factors. Because mutations in the RRF motif result in constitutive gene expression throughout the cell cycle, this sequence is likely to be the binding site for a cell cycle-regulated transcriptional repressor. Consistent with this hypothesis, Caulobacter extracts contain an activity that binds specifically to the RRF in vitro.

Original languageEnglish (US)
Pages (from-to)4860-4865
Number of pages6
JournalJournal of Bacteriology
Volume183
Issue number16
DOIs
StatePublished - Aug 15 2001

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Caulobacter
Cell Cycle
Caulobacter crescentus
Gene Expression
cdc Genes
Mutation
Gene Regulatory Networks
Phase Transition
G1 Phase
DNA Replication
S Phase
Binding Sites
Bacteria
Genes

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

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title = "Conserved promoter motif is required for cell cycle timing of dnaX transcription in Caulobacter",
abstract = "Cells use highly regulated transcriptional networks to control temporally regulated events. In the bacterium Caulobacter crescentus, many cellular processes are temporally regulated with respect to the cell cycle, and the genes required for these processes are expressed immediately before the products are needed. Genes encoding factors required for DNA replication, including dnaX, dnaA, dnaN, gyrB, and dnaK, are induced at the G1/S-phase transition. By analyzing mutations in the dnax promoter, we identified a motif between the -10 and -35 regions that is required for proper timing of gene expression. This motif, named RRF (for repression of replication factors), is conserved in the promoters of other coordinately induced replication factors. Because mutations in the RRF motif result in constitutive gene expression throughout the cell cycle, this sequence is likely to be the binding site for a cell cycle-regulated transcriptional repressor. Consistent with this hypothesis, Caulobacter extracts contain an activity that binds specifically to the RRF in vitro.",
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Conserved promoter motif is required for cell cycle timing of dnaX transcription in Caulobacter. / Keiler, Kenneth Charles; Shapiro, L.

In: Journal of Bacteriology, Vol. 183, No. 16, 15.08.2001, p. 4860-4865.

Research output: Contribution to journalArticle

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AU - Keiler, Kenneth Charles

AU - Shapiro, L.

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AB - Cells use highly regulated transcriptional networks to control temporally regulated events. In the bacterium Caulobacter crescentus, many cellular processes are temporally regulated with respect to the cell cycle, and the genes required for these processes are expressed immediately before the products are needed. Genes encoding factors required for DNA replication, including dnaX, dnaA, dnaN, gyrB, and dnaK, are induced at the G1/S-phase transition. By analyzing mutations in the dnax promoter, we identified a motif between the -10 and -35 regions that is required for proper timing of gene expression. This motif, named RRF (for repression of replication factors), is conserved in the promoters of other coordinately induced replication factors. Because mutations in the RRF motif result in constitutive gene expression throughout the cell cycle, this sequence is likely to be the binding site for a cell cycle-regulated transcriptional repressor. Consistent with this hypothesis, Caulobacter extracts contain an activity that binds specifically to the RRF in vitro.

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