Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome

Lalitha Nagarajan, Jiri Zavadil, David Claxton, Xiaoyan Lu, Jeff Fairman, Janet A. Warrington, John J. Wasmuth, A. Craig Chinault, Cordelia E. Sever, Marilyn L. Slovak, Cheryl L. Willman, Albert B. Deisseroth

Research output: Contribution to journalArticle

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Abstract

Interstitial deletions of the long arm of chromosome 5 are common in a number of disorders of leukemic and preleukemic myeloid disorders. Although the limits of these deletions vary among patients, a region of cytogenetic overlap that includes band 5q31 is deleted consistently, suggesting loss of 5q31 loci critical for normal myeloid differentiation and leukemogenesis. An anonymous genomic DNA segment D5S89, previously mapped to 5q21-31, detects consistent loss of alleles in cases showing the 5q- chromosome at presentation or relapse. Analysis of a panel of natural-deletion somatic- cell hybrids in conjunction with irradiation hybrids containing fragments of human chromosome 5q shows that the D5S89 locus is telomeric to the interleukin (IL) genes (IL-3, IL-4, IL-5, IL-9, and granulocyte-macrophage colony-stimulating factor [GM-CSF]) and interferon response factor-1 (IRF-1) gene and centromeric to the early response transcription factor (early growth response gene-1 [EGR-1]) on 5q31. To further define the principal region of loss, we have isolated and characterized yeast artificial chromosomes (YACs) spanning D5S89. The presence of several CpG islands within the 300-kb YAC is suggestive of multiple transcription units. However, IL-4, IL-5, IRF-1, IL- 3, GM-CSF, and EGR-1 genes were not detected in the YAC clone spanning D5S89, implying that none of these genes are in the vicinity of the D5S89 marker. Further characterization of these YACs should facilitate the isolation of novel candidate genes that may play a role in the evolution of the abnormal phenotype associated with 5q- chromosome.

Original languageEnglish (US)
Pages (from-to)199-208
Number of pages10
JournalBlood
Volume83
Issue number1
StatePublished - Jan 1 1994

Fingerprint

Interleukins
Chromosomes
Multigene Family
Yeast Artificial Chromosomes
Genes
Growth
Yeast
Early Growth Response Transcription Factors
Interleukin-3
Interleukin-5
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-4
Interferons
Early Growth Response Protein 1
Interleukin-9
Chromosomes, Human, Pair 5
CpG Islands
Hybrid Cells
Human Chromosomes
Cytogenetics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Nagarajan, L., Zavadil, J., Claxton, D., Lu, X., Fairman, J., Warrington, J. A., ... Deisseroth, A. B. (1994). Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome. Blood, 83(1), 199-208.
Nagarajan, Lalitha ; Zavadil, Jiri ; Claxton, David ; Lu, Xiaoyan ; Fairman, Jeff ; Warrington, Janet A. ; Wasmuth, John J. ; Chinault, A. Craig ; Sever, Cordelia E. ; Slovak, Marilyn L. ; Willman, Cheryl L. ; Deisseroth, Albert B. / Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome. In: Blood. 1994 ; Vol. 83, No. 1. pp. 199-208.
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title = "Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome",
abstract = "Interstitial deletions of the long arm of chromosome 5 are common in a number of disorders of leukemic and preleukemic myeloid disorders. Although the limits of these deletions vary among patients, a region of cytogenetic overlap that includes band 5q31 is deleted consistently, suggesting loss of 5q31 loci critical for normal myeloid differentiation and leukemogenesis. An anonymous genomic DNA segment D5S89, previously mapped to 5q21-31, detects consistent loss of alleles in cases showing the 5q- chromosome at presentation or relapse. Analysis of a panel of natural-deletion somatic- cell hybrids in conjunction with irradiation hybrids containing fragments of human chromosome 5q shows that the D5S89 locus is telomeric to the interleukin (IL) genes (IL-3, IL-4, IL-5, IL-9, and granulocyte-macrophage colony-stimulating factor [GM-CSF]) and interferon response factor-1 (IRF-1) gene and centromeric to the early response transcription factor (early growth response gene-1 [EGR-1]) on 5q31. To further define the principal region of loss, we have isolated and characterized yeast artificial chromosomes (YACs) spanning D5S89. The presence of several CpG islands within the 300-kb YAC is suggestive of multiple transcription units. However, IL-4, IL-5, IRF-1, IL- 3, GM-CSF, and EGR-1 genes were not detected in the YAC clone spanning D5S89, implying that none of these genes are in the vicinity of the D5S89 marker. Further characterization of these YACs should facilitate the isolation of novel candidate genes that may play a role in the evolution of the abnormal phenotype associated with 5q- chromosome.",
author = "Lalitha Nagarajan and Jiri Zavadil and David Claxton and Xiaoyan Lu and Jeff Fairman and Warrington, {Janet A.} and Wasmuth, {John J.} and Chinault, {A. Craig} and Sever, {Cordelia E.} and Slovak, {Marilyn L.} and Willman, {Cheryl L.} and Deisseroth, {Albert B.}",
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Nagarajan, L, Zavadil, J, Claxton, D, Lu, X, Fairman, J, Warrington, JA, Wasmuth, JJ, Chinault, AC, Sever, CE, Slovak, ML, Willman, CL & Deisseroth, AB 1994, 'Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome', Blood, vol. 83, no. 1, pp. 199-208.

Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome. / Nagarajan, Lalitha; Zavadil, Jiri; Claxton, David; Lu, Xiaoyan; Fairman, Jeff; Warrington, Janet A.; Wasmuth, John J.; Chinault, A. Craig; Sever, Cordelia E.; Slovak, Marilyn L.; Willman, Cheryl L.; Deisseroth, Albert B.

In: Blood, Vol. 83, No. 1, 01.01.1994, p. 199-208.

Research output: Contribution to journalArticle

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T1 - Consistent loss of the D5S89 locus mapping telomeric to the interleukin gene cluster and centromeric to EGR-1 in patients with 5q- chromosome

AU - Nagarajan, Lalitha

AU - Zavadil, Jiri

AU - Claxton, David

AU - Lu, Xiaoyan

AU - Fairman, Jeff

AU - Warrington, Janet A.

AU - Wasmuth, John J.

AU - Chinault, A. Craig

AU - Sever, Cordelia E.

AU - Slovak, Marilyn L.

AU - Willman, Cheryl L.

AU - Deisseroth, Albert B.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Interstitial deletions of the long arm of chromosome 5 are common in a number of disorders of leukemic and preleukemic myeloid disorders. Although the limits of these deletions vary among patients, a region of cytogenetic overlap that includes band 5q31 is deleted consistently, suggesting loss of 5q31 loci critical for normal myeloid differentiation and leukemogenesis. An anonymous genomic DNA segment D5S89, previously mapped to 5q21-31, detects consistent loss of alleles in cases showing the 5q- chromosome at presentation or relapse. Analysis of a panel of natural-deletion somatic- cell hybrids in conjunction with irradiation hybrids containing fragments of human chromosome 5q shows that the D5S89 locus is telomeric to the interleukin (IL) genes (IL-3, IL-4, IL-5, IL-9, and granulocyte-macrophage colony-stimulating factor [GM-CSF]) and interferon response factor-1 (IRF-1) gene and centromeric to the early response transcription factor (early growth response gene-1 [EGR-1]) on 5q31. To further define the principal region of loss, we have isolated and characterized yeast artificial chromosomes (YACs) spanning D5S89. The presence of several CpG islands within the 300-kb YAC is suggestive of multiple transcription units. However, IL-4, IL-5, IRF-1, IL- 3, GM-CSF, and EGR-1 genes were not detected in the YAC clone spanning D5S89, implying that none of these genes are in the vicinity of the D5S89 marker. Further characterization of these YACs should facilitate the isolation of novel candidate genes that may play a role in the evolution of the abnormal phenotype associated with 5q- chromosome.

AB - Interstitial deletions of the long arm of chromosome 5 are common in a number of disorders of leukemic and preleukemic myeloid disorders. Although the limits of these deletions vary among patients, a region of cytogenetic overlap that includes band 5q31 is deleted consistently, suggesting loss of 5q31 loci critical for normal myeloid differentiation and leukemogenesis. An anonymous genomic DNA segment D5S89, previously mapped to 5q21-31, detects consistent loss of alleles in cases showing the 5q- chromosome at presentation or relapse. Analysis of a panel of natural-deletion somatic- cell hybrids in conjunction with irradiation hybrids containing fragments of human chromosome 5q shows that the D5S89 locus is telomeric to the interleukin (IL) genes (IL-3, IL-4, IL-5, IL-9, and granulocyte-macrophage colony-stimulating factor [GM-CSF]) and interferon response factor-1 (IRF-1) gene and centromeric to the early response transcription factor (early growth response gene-1 [EGR-1]) on 5q31. To further define the principal region of loss, we have isolated and characterized yeast artificial chromosomes (YACs) spanning D5S89. The presence of several CpG islands within the 300-kb YAC is suggestive of multiple transcription units. However, IL-4, IL-5, IRF-1, IL- 3, GM-CSF, and EGR-1 genes were not detected in the YAC clone spanning D5S89, implying that none of these genes are in the vicinity of the D5S89 marker. Further characterization of these YACs should facilitate the isolation of novel candidate genes that may play a role in the evolution of the abnormal phenotype associated with 5q- chromosome.

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