Construction and characterization of hybrid polyomavirus genomes

W. F. Chuke, D. L. Walker, L. B. Peitzman, Richard John Frisque

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Several studies have suggested that certain unique features of the JC virus (JCV) regulatory region are responsible for the restricted lytic and transforming activities of this virus in vitro. To pursue this possibility, we have constructed hybrid polyomavirus genomes by exchanging the regulatory sequences of JCV, BK virus (BKV), and simian virus 40 (SV40). The host range of JCV was not expanded by the substitution of the BKV or SV40 regulatory signals; such hybrids were nonviable even in primary human fetal glial cells, the sole permissive cell for JCV. However, chimeric DNAs containing JCV regulatory sequences and BKV- or SV40-coding sequences were lytically active, indicating that the BKV and SV40 T proteins were capable of effectively interacting with JCV replication and transcription signals to the restricted lytic activity of this virus, it appears that the latter sequences, most likely those encoding the T proteins, have a greater influence on this behavior.

Original languageEnglish (US)
Pages (from-to)960-971
Number of pages12
JournalJournal of virology
Volume60
Issue number3
StatePublished - Dec 1 1986

Fingerprint

JC polyomavirus
Polyomavirus
JC Virus
Simian virus 40
BK Virus
Genome
viruses
genome
regulatory sequences
Viruses
Nucleic Acid Regulatory Sequences
Host Specificity
neuroglia
Virus Replication
virus replication
Neuroglia
host range
Proteins
proteins
transcription (genetics)

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Chuke, W. F., Walker, D. L., Peitzman, L. B., & Frisque, R. J. (1986). Construction and characterization of hybrid polyomavirus genomes. Journal of virology, 60(3), 960-971.
Chuke, W. F. ; Walker, D. L. ; Peitzman, L. B. ; Frisque, Richard John. / Construction and characterization of hybrid polyomavirus genomes. In: Journal of virology. 1986 ; Vol. 60, No. 3. pp. 960-971.
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Chuke, WF, Walker, DL, Peitzman, LB & Frisque, RJ 1986, 'Construction and characterization of hybrid polyomavirus genomes', Journal of virology, vol. 60, no. 3, pp. 960-971.

Construction and characterization of hybrid polyomavirus genomes. / Chuke, W. F.; Walker, D. L.; Peitzman, L. B.; Frisque, Richard John.

In: Journal of virology, Vol. 60, No. 3, 01.12.1986, p. 960-971.

Research output: Contribution to journalArticle

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Chuke WF, Walker DL, Peitzman LB, Frisque RJ. Construction and characterization of hybrid polyomavirus genomes. Journal of virology. 1986 Dec 1;60(3):960-971.