Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: A new resource for plant comparative genomics

Wenming Wang, Milos Tanurdzic, Meizhong Luo, Nicholas Sisneros, Ran Kim Hye, Jing Ke Weng, Dave Kudrna, Christopher Mueller, K. Arumuganathan, John Edward Carlson, Clint Chapple, Claude Walker Depamphilis, Dina Mandoli, Jeff Tomkins, Rod A. Wing, Jo Ann Banks

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Background: The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants. Results: Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes. Conclusion: The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.

Original languageEnglish (US)
Article number10
JournalBMC plant biology
Volume5
DOIs
StatePublished - Jun 14 2005

Fingerprint

Selaginella
bacterial artificial chromosomes
genomics
genome
Huperzia
clones
Isoetes
Spermatophytina
nucleic acid hybridization
vascular plants
flow cytometry
chloroplasts
phylogeny
genes
cells

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Wang, Wenming ; Tanurdzic, Milos ; Luo, Meizhong ; Sisneros, Nicholas ; Hye, Ran Kim ; Weng, Jing Ke ; Kudrna, Dave ; Mueller, Christopher ; Arumuganathan, K. ; Carlson, John Edward ; Chapple, Clint ; Depamphilis, Claude Walker ; Mandoli, Dina ; Tomkins, Jeff ; Wing, Rod A. ; Banks, Jo Ann. / Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii : A new resource for plant comparative genomics. In: BMC plant biology. 2005 ; Vol. 5.
@article{884422ffe9ef4d13954f524068fac81c,
title = "Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: A new resource for plant comparative genomics",
abstract = "Background: The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants. Results: Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3{\%} of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes. Conclusion: The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.",
author = "Wenming Wang and Milos Tanurdzic and Meizhong Luo and Nicholas Sisneros and Hye, {Ran Kim} and Weng, {Jing Ke} and Dave Kudrna and Christopher Mueller and K. Arumuganathan and Carlson, {John Edward} and Clint Chapple and Depamphilis, {Claude Walker} and Dina Mandoli and Jeff Tomkins and Wing, {Rod A.} and Banks, {Jo Ann}",
year = "2005",
month = "6",
day = "14",
doi = "10.1186/1471-2229-5-10",
language = "English (US)",
volume = "5",
journal = "BMC Plant Biology",
issn = "1471-2229",
publisher = "BioMed Central",

}

Wang, W, Tanurdzic, M, Luo, M, Sisneros, N, Hye, RK, Weng, JK, Kudrna, D, Mueller, C, Arumuganathan, K, Carlson, JE, Chapple, C, Depamphilis, CW, Mandoli, D, Tomkins, J, Wing, RA & Banks, JA 2005, 'Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii: A new resource for plant comparative genomics', BMC plant biology, vol. 5, 10. https://doi.org/10.1186/1471-2229-5-10

Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii : A new resource for plant comparative genomics. / Wang, Wenming; Tanurdzic, Milos; Luo, Meizhong; Sisneros, Nicholas; Hye, Ran Kim; Weng, Jing Ke; Kudrna, Dave; Mueller, Christopher; Arumuganathan, K.; Carlson, John Edward; Chapple, Clint; Depamphilis, Claude Walker; Mandoli, Dina; Tomkins, Jeff; Wing, Rod A.; Banks, Jo Ann.

In: BMC plant biology, Vol. 5, 10, 14.06.2005.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Construction of a bacterial artificial chromosome library from the spikemoss Selaginella moellendorffii

T2 - A new resource for plant comparative genomics

AU - Wang, Wenming

AU - Tanurdzic, Milos

AU - Luo, Meizhong

AU - Sisneros, Nicholas

AU - Hye, Ran Kim

AU - Weng, Jing Ke

AU - Kudrna, Dave

AU - Mueller, Christopher

AU - Arumuganathan, K.

AU - Carlson, John Edward

AU - Chapple, Clint

AU - Depamphilis, Claude Walker

AU - Mandoli, Dina

AU - Tomkins, Jeff

AU - Wing, Rod A.

AU - Banks, Jo Ann

PY - 2005/6/14

Y1 - 2005/6/14

N2 - Background: The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants. Results: Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes. Conclusion: The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.

AB - Background: The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants. Results: Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes. Conclusion: The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.

UR - http://www.scopus.com/inward/record.url?scp=23044470256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23044470256&partnerID=8YFLogxK

U2 - 10.1186/1471-2229-5-10

DO - 10.1186/1471-2229-5-10

M3 - Article

C2 - 15955246

AN - SCOPUS:23044470256

VL - 5

JO - BMC Plant Biology

JF - BMC Plant Biology

SN - 1471-2229

M1 - 10

ER -