Construction of graphene-based enzyme system for rapid photo-assisted proteolysis

G. Cheng, S. J. Hao, Siyang Zheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Rapid and efficient protein digestion by sequence-specific proteases is a prerequisite and critical step in proteomics. A novel photo-assisted nanoreactor was designed and constructed for rapid protein digestion. Porous graphene-silica composite material was prepared, and trypsin molecules were further immobilized in the nanopores to construct the functional nanoreactor for protein digestion. By taking advantage of the unique response of graphene to the near infrared light, this nanoreactor can realized rapid protein digestion under near infrared laser radiation, which is much faster than the widely applied overnight in-solution digestion using free trypsin molecules. It's expected that this work would contribute to the rapid and high throughput protein digestion in proteomics.

Original languageEnglish (US)
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1374-1377
Number of pages4
ISBN (Electronic)9781479989553
DOIs
StatePublished - Aug 5 2015
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: Jun 21 2015Jun 25 2015

Publication series

Name2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
CountryUnited States
CityAnchorage
Period6/21/156/25/15

Fingerprint

Proteolysis
Graphene
enzymes
Nanoreactors
graphene
Enzymes
proteins
Proteins
trypsin
Infrared radiation
protease
Molecules
Nanopores
Infrared lasers
Laser radiation
infrared lasers
molecules
Silica
Throughput
laser beams

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Cheng, G., Hao, S. J., & Zheng, S. (2015). Construction of graphene-based enzyme system for rapid photo-assisted proteolysis. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 1374-1377). [7181188] (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181188
Cheng, G. ; Hao, S. J. ; Zheng, Siyang. / Construction of graphene-based enzyme system for rapid photo-assisted proteolysis. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 1374-1377 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).
@inproceedings{b76f8795dba84dc784c17f92e390342e,
title = "Construction of graphene-based enzyme system for rapid photo-assisted proteolysis",
abstract = "Rapid and efficient protein digestion by sequence-specific proteases is a prerequisite and critical step in proteomics. A novel photo-assisted nanoreactor was designed and constructed for rapid protein digestion. Porous graphene-silica composite material was prepared, and trypsin molecules were further immobilized in the nanopores to construct the functional nanoreactor for protein digestion. By taking advantage of the unique response of graphene to the near infrared light, this nanoreactor can realized rapid protein digestion under near infrared laser radiation, which is much faster than the widely applied overnight in-solution digestion using free trypsin molecules. It's expected that this work would contribute to the rapid and high throughput protein digestion in proteomics.",
author = "G. Cheng and Hao, {S. J.} and Siyang Zheng",
year = "2015",
month = "8",
day = "5",
doi = "10.1109/TRANSDUCERS.2015.7181188",
language = "English (US)",
series = "2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
pages = "1374--1377",
booktitle = "2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015",
address = "United States",

}

Cheng, G, Hao, SJ & Zheng, S 2015, Construction of graphene-based enzyme system for rapid photo-assisted proteolysis. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181188, 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Institute of Electrical and Electronics Engineers Inc., pp. 1374-1377, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 6/21/15. https://doi.org/10.1109/TRANSDUCERS.2015.7181188

Construction of graphene-based enzyme system for rapid photo-assisted proteolysis. / Cheng, G.; Hao, S. J.; Zheng, Siyang.

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 1374-1377 7181188 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Construction of graphene-based enzyme system for rapid photo-assisted proteolysis

AU - Cheng, G.

AU - Hao, S. J.

AU - Zheng, Siyang

PY - 2015/8/5

Y1 - 2015/8/5

N2 - Rapid and efficient protein digestion by sequence-specific proteases is a prerequisite and critical step in proteomics. A novel photo-assisted nanoreactor was designed and constructed for rapid protein digestion. Porous graphene-silica composite material was prepared, and trypsin molecules were further immobilized in the nanopores to construct the functional nanoreactor for protein digestion. By taking advantage of the unique response of graphene to the near infrared light, this nanoreactor can realized rapid protein digestion under near infrared laser radiation, which is much faster than the widely applied overnight in-solution digestion using free trypsin molecules. It's expected that this work would contribute to the rapid and high throughput protein digestion in proteomics.

AB - Rapid and efficient protein digestion by sequence-specific proteases is a prerequisite and critical step in proteomics. A novel photo-assisted nanoreactor was designed and constructed for rapid protein digestion. Porous graphene-silica composite material was prepared, and trypsin molecules were further immobilized in the nanopores to construct the functional nanoreactor for protein digestion. By taking advantage of the unique response of graphene to the near infrared light, this nanoreactor can realized rapid protein digestion under near infrared laser radiation, which is much faster than the widely applied overnight in-solution digestion using free trypsin molecules. It's expected that this work would contribute to the rapid and high throughput protein digestion in proteomics.

UR - http://www.scopus.com/inward/record.url?scp=84955472845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84955472845&partnerID=8YFLogxK

U2 - 10.1109/TRANSDUCERS.2015.7181188

DO - 10.1109/TRANSDUCERS.2015.7181188

M3 - Conference contribution

AN - SCOPUS:84955472845

T3 - 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

SP - 1374

EP - 1377

BT - 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

PB - Institute of Electrical and Electronics Engineers Inc.

ER -

Cheng G, Hao SJ, Zheng S. Construction of graphene-based enzyme system for rapid photo-assisted proteolysis. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 1374-1377. 7181188. (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). https://doi.org/10.1109/TRANSDUCERS.2015.7181188