Construction of graphene-based enzyme system for rapid photo-assisted proteolysis

G. Cheng, S. J. Hao, S. Y. Zheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Rapid and efficient protein digestion by sequence-specific proteases is a prerequisite and critical step in proteomics. A novel photo-assisted nanoreactor was designed and constructed for rapid protein digestion. Porous graphene-silica composite material was prepared, and trypsin molecules were further immobilized in the nanopores to construct the functional nanoreactor for protein digestion. By taking advantage of the unique response of graphene to the near infrared light, this nanoreactor can realized rapid protein digestion under near infrared laser radiation, which is much faster than the widely applied overnight in-solution digestion using free trypsin molecules. It's expected that this work would contribute to the rapid and high throughput protein digestion in proteomics.

Original languageEnglish (US)
Title of host publication2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1374-1377
Number of pages4
ISBN (Electronic)9781479989553
DOIs
StatePublished - Aug 5 2015
Event18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States
Duration: Jun 21 2015Jun 25 2015

Publication series

Name2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Other

Other18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
CountryUnited States
CityAnchorage
Period6/21/156/25/15

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Electrical and Electronic Engineering

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    Cheng, G., Hao, S. J., & Zheng, S. Y. (2015). Construction of graphene-based enzyme system for rapid photo-assisted proteolysis. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 1374-1377). [7181188] (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181188