Contact activation of the plasma coagulation cascade. I. Procoagulant surface chemistry and energy

Erwin A. Vogler, Jane C. Graper, Garry R. Harper, Harry W. Sugg, Lorraine M. Lander, William J. Brittain

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Contact activation of the intrinsic pathway of porcine blood plasma coagulation is shown to be a steep exponential‐like function of procoagulant surface energy, with low activation observed for poorly water‐wettable surfaces and very high activation for fully water‐wettable surfaces. Test procoagulants studied were a system of oxidized polystyrene films with varying wettability (surface energy) and glass discs bearing close‐packed self‐assembled silane monolayers (SAMs) with well‐defined chemistry consisting of 12 different terminating chemical functionalities. A monotonic trend of increasing coagulation activation with increasing water wettability was observed for the oxidized polystyrene system whereas results with SAM procoagulants suggested a level of chemical specificity over and above the surface energy trend. In particular, it was noted that coagulation activation by SAMs terminated with CO2H was much higher than anticipated based on surface wettability whereas NH3⊕‐terminated SAMs exhibited very low procoagulant activity. SAMs terminated in (CH2)2(CF2)7CF3 behaved as anticipated based on surface energy with very low procoagulant activity and did not exhibit special properties sometimes attributed to perfluorinated compounds. Quantitative ranking of the inherent coagulation activation properties of procoagulant surfaces was obtained by application of a straightforward phenomenological model expressed in a closed‐form mathematical equation relating coagulation time to procoagulant surface area. Fit of the model with a single adjustable parameter to experimental measurements of porcine platelet‐poor plasma coagulation time was very good, implying that assertions and simplifications of the model adequately simulated reality. Two important propositions of the model were that (1) the number of putative “activating sites” scaled linearly with procoagulant surface area, and (2) contact activation of the plasma coagulation cascade was catalytic in the sense that these activating sites were not consumed or “poisoned” by irreversible or slowly reversible protein adsorption during coagulation. An extension of the coagulation model proposed that procoagulant activation properties scale exponentially with the surface density of polar (acid‐base) sites, which, in turn, was related to procoagulant wettability. © 1995 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)1005-1016
Number of pages12
JournalJournal of Biomedical Materials Research
Volume29
Issue number8
DOIs
StatePublished - Jan 1 1995

Fingerprint

Coagulation
Surface chemistry
Interfacial energy
Chemical activation
Silanes
Plasmas
Monolayers
Wetting
Polystyrenes
Bearings (structural)
Blood
Proteins
Adsorption
Glass
Water

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Vogler, E. A., Graper, J. C., Harper, G. R., Sugg, H. W., Lander, L. M., & Brittain, W. J. (1995). Contact activation of the plasma coagulation cascade. I. Procoagulant surface chemistry and energy. Journal of Biomedical Materials Research, 29(8), 1005-1016. https://doi.org/10.1002/jbm.820290813
Vogler, Erwin A. ; Graper, Jane C. ; Harper, Garry R. ; Sugg, Harry W. ; Lander, Lorraine M. ; Brittain, William J. / Contact activation of the plasma coagulation cascade. I. Procoagulant surface chemistry and energy. In: Journal of Biomedical Materials Research. 1995 ; Vol. 29, No. 8. pp. 1005-1016.
@article{2c9a15c9f8ad4857bfc188a973f092e1,
title = "Contact activation of the plasma coagulation cascade. I. Procoagulant surface chemistry and energy",
abstract = "Contact activation of the intrinsic pathway of porcine blood plasma coagulation is shown to be a steep exponential‐like function of procoagulant surface energy, with low activation observed for poorly water‐wettable surfaces and very high activation for fully water‐wettable surfaces. Test procoagulants studied were a system of oxidized polystyrene films with varying wettability (surface energy) and glass discs bearing close‐packed self‐assembled silane monolayers (SAMs) with well‐defined chemistry consisting of 12 different terminating chemical functionalities. A monotonic trend of increasing coagulation activation with increasing water wettability was observed for the oxidized polystyrene system whereas results with SAM procoagulants suggested a level of chemical specificity over and above the surface energy trend. In particular, it was noted that coagulation activation by SAMs terminated with CO2H was much higher than anticipated based on surface wettability whereas NH3⊕‐terminated SAMs exhibited very low procoagulant activity. SAMs terminated in (CH2)2(CF2)7CF3 behaved as anticipated based on surface energy with very low procoagulant activity and did not exhibit special properties sometimes attributed to perfluorinated compounds. Quantitative ranking of the inherent coagulation activation properties of procoagulant surfaces was obtained by application of a straightforward phenomenological model expressed in a closed‐form mathematical equation relating coagulation time to procoagulant surface area. Fit of the model with a single adjustable parameter to experimental measurements of porcine platelet‐poor plasma coagulation time was very good, implying that assertions and simplifications of the model adequately simulated reality. Two important propositions of the model were that (1) the number of putative “activating sites” scaled linearly with procoagulant surface area, and (2) contact activation of the plasma coagulation cascade was catalytic in the sense that these activating sites were not consumed or “poisoned” by irreversible or slowly reversible protein adsorption during coagulation. An extension of the coagulation model proposed that procoagulant activation properties scale exponentially with the surface density of polar (acid‐base) sites, which, in turn, was related to procoagulant wettability. {\circledC} 1995 John Wiley & Sons, Inc.",
author = "Vogler, {Erwin A.} and Graper, {Jane C.} and Harper, {Garry R.} and Sugg, {Harry W.} and Lander, {Lorraine M.} and Brittain, {William J.}",
year = "1995",
month = "1",
day = "1",
doi = "10.1002/jbm.820290813",
language = "English (US)",
volume = "29",
pages = "1005--1016",
journal = "Journal of Biomedical Materials Research",
issn = "0021-9304",
publisher = "Heterocorporation",
number = "8",

}

Contact activation of the plasma coagulation cascade. I. Procoagulant surface chemistry and energy. / Vogler, Erwin A.; Graper, Jane C.; Harper, Garry R.; Sugg, Harry W.; Lander, Lorraine M.; Brittain, William J.

In: Journal of Biomedical Materials Research, Vol. 29, No. 8, 01.01.1995, p. 1005-1016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Contact activation of the plasma coagulation cascade. I. Procoagulant surface chemistry and energy

AU - Vogler, Erwin A.

AU - Graper, Jane C.

AU - Harper, Garry R.

AU - Sugg, Harry W.

AU - Lander, Lorraine M.

AU - Brittain, William J.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Contact activation of the intrinsic pathway of porcine blood plasma coagulation is shown to be a steep exponential‐like function of procoagulant surface energy, with low activation observed for poorly water‐wettable surfaces and very high activation for fully water‐wettable surfaces. Test procoagulants studied were a system of oxidized polystyrene films with varying wettability (surface energy) and glass discs bearing close‐packed self‐assembled silane monolayers (SAMs) with well‐defined chemistry consisting of 12 different terminating chemical functionalities. A monotonic trend of increasing coagulation activation with increasing water wettability was observed for the oxidized polystyrene system whereas results with SAM procoagulants suggested a level of chemical specificity over and above the surface energy trend. In particular, it was noted that coagulation activation by SAMs terminated with CO2H was much higher than anticipated based on surface wettability whereas NH3⊕‐terminated SAMs exhibited very low procoagulant activity. SAMs terminated in (CH2)2(CF2)7CF3 behaved as anticipated based on surface energy with very low procoagulant activity and did not exhibit special properties sometimes attributed to perfluorinated compounds. Quantitative ranking of the inherent coagulation activation properties of procoagulant surfaces was obtained by application of a straightforward phenomenological model expressed in a closed‐form mathematical equation relating coagulation time to procoagulant surface area. Fit of the model with a single adjustable parameter to experimental measurements of porcine platelet‐poor plasma coagulation time was very good, implying that assertions and simplifications of the model adequately simulated reality. Two important propositions of the model were that (1) the number of putative “activating sites” scaled linearly with procoagulant surface area, and (2) contact activation of the plasma coagulation cascade was catalytic in the sense that these activating sites were not consumed or “poisoned” by irreversible or slowly reversible protein adsorption during coagulation. An extension of the coagulation model proposed that procoagulant activation properties scale exponentially with the surface density of polar (acid‐base) sites, which, in turn, was related to procoagulant wettability. © 1995 John Wiley & Sons, Inc.

AB - Contact activation of the intrinsic pathway of porcine blood plasma coagulation is shown to be a steep exponential‐like function of procoagulant surface energy, with low activation observed for poorly water‐wettable surfaces and very high activation for fully water‐wettable surfaces. Test procoagulants studied were a system of oxidized polystyrene films with varying wettability (surface energy) and glass discs bearing close‐packed self‐assembled silane monolayers (SAMs) with well‐defined chemistry consisting of 12 different terminating chemical functionalities. A monotonic trend of increasing coagulation activation with increasing water wettability was observed for the oxidized polystyrene system whereas results with SAM procoagulants suggested a level of chemical specificity over and above the surface energy trend. In particular, it was noted that coagulation activation by SAMs terminated with CO2H was much higher than anticipated based on surface wettability whereas NH3⊕‐terminated SAMs exhibited very low procoagulant activity. SAMs terminated in (CH2)2(CF2)7CF3 behaved as anticipated based on surface energy with very low procoagulant activity and did not exhibit special properties sometimes attributed to perfluorinated compounds. Quantitative ranking of the inherent coagulation activation properties of procoagulant surfaces was obtained by application of a straightforward phenomenological model expressed in a closed‐form mathematical equation relating coagulation time to procoagulant surface area. Fit of the model with a single adjustable parameter to experimental measurements of porcine platelet‐poor plasma coagulation time was very good, implying that assertions and simplifications of the model adequately simulated reality. Two important propositions of the model were that (1) the number of putative “activating sites” scaled linearly with procoagulant surface area, and (2) contact activation of the plasma coagulation cascade was catalytic in the sense that these activating sites were not consumed or “poisoned” by irreversible or slowly reversible protein adsorption during coagulation. An extension of the coagulation model proposed that procoagulant activation properties scale exponentially with the surface density of polar (acid‐base) sites, which, in turn, was related to procoagulant wettability. © 1995 John Wiley & Sons, Inc.

UR - http://www.scopus.com/inward/record.url?scp=0029347516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029347516&partnerID=8YFLogxK

U2 - 10.1002/jbm.820290813

DO - 10.1002/jbm.820290813

M3 - Article

C2 - 7593031

AN - SCOPUS:0029347516

VL - 29

SP - 1005

EP - 1016

JO - Journal of Biomedical Materials Research

JF - Journal of Biomedical Materials Research

SN - 0021-9304

IS - 8

ER -