Contaminant exposure, biochemical, and histopathological biomarkers in white suckers from contaminated and reference sites in the Sheboygan River, Wisconsin

Candy S. Schrank, Susan M. Cormier, Vicki Suzette Blazer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Fish populations of the lower Sheboygan River, located in east-central Wisconsin, are considered impaired under the Great Lakes Water Quality Agreement between the United States and Canada. Pollutants in the Sheboygan River system include: polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and heavy metals. This study compared general health and biochemical parameters, histology of selected organs, and contaminant residues and metabolites in a population of white suckers (Catostomus commersoni) from u contaminated reach of the Sheboygan River and an upstream reference site. Fish from the contaminated site had significantly lower hematocrits, significantly induced ethoxyresorufin-O-deethylase (EROD) activity as a measurement of hepatic mixed-function oxygenase activity, higher biliary metabolites of PAHs, and higher tissue concentrations of PCBs and p,p'-DDE relative to an upstream reference population. Analysis of covuriance suggests that both PCB and PAH exposure may be influencing EROD activities. Fish from the contaminated site featured more basophilic clusters and developing nephrons in kidney tissue suggesting the presence of a nephrotoxicant. Also, more fish exhibited hepatic lesions including diffuse cellular vacuolation, multifocal coagulative necrosis, bile ductal hyperplasia, and foci of cellular alteration which may be biomarkers for contaminant impacts. This study demonstrates that white suckers residing in the lower reaches of the Sheboygan River absorbed significant amounts of PAHs and PCBs and also exhibited hematological, biochemical and histological alterations some of which suggest impaired fish condition.

Original languageEnglish (US)
Pages (from-to)119-130
Number of pages12
JournalJournal of Great Lakes Research
Volume23
Issue number2
DOIs
StatePublished - Jan 1 1997

Fingerprint

Wisconsin River
polychlorinated biphenyls
biomarker
polycyclic aromatic hydrocarbons
biomarkers
PCB
PAH
pollutant
fish
rivers
river
metabolite
metabolites
Catostomus
mixed function oxidase
general health
DDE (pesticide)
nephrons
liver
histology

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

Cite this

@article{4d4ebd5ff1ba43e780607831a8ea21c5,
title = "Contaminant exposure, biochemical, and histopathological biomarkers in white suckers from contaminated and reference sites in the Sheboygan River, Wisconsin",
abstract = "Fish populations of the lower Sheboygan River, located in east-central Wisconsin, are considered impaired under the Great Lakes Water Quality Agreement between the United States and Canada. Pollutants in the Sheboygan River system include: polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and heavy metals. This study compared general health and biochemical parameters, histology of selected organs, and contaminant residues and metabolites in a population of white suckers (Catostomus commersoni) from u contaminated reach of the Sheboygan River and an upstream reference site. Fish from the contaminated site had significantly lower hematocrits, significantly induced ethoxyresorufin-O-deethylase (EROD) activity as a measurement of hepatic mixed-function oxygenase activity, higher biliary metabolites of PAHs, and higher tissue concentrations of PCBs and p,p'-DDE relative to an upstream reference population. Analysis of covuriance suggests that both PCB and PAH exposure may be influencing EROD activities. Fish from the contaminated site featured more basophilic clusters and developing nephrons in kidney tissue suggesting the presence of a nephrotoxicant. Also, more fish exhibited hepatic lesions including diffuse cellular vacuolation, multifocal coagulative necrosis, bile ductal hyperplasia, and foci of cellular alteration which may be biomarkers for contaminant impacts. This study demonstrates that white suckers residing in the lower reaches of the Sheboygan River absorbed significant amounts of PAHs and PCBs and also exhibited hematological, biochemical and histological alterations some of which suggest impaired fish condition.",
author = "Schrank, {Candy S.} and Cormier, {Susan M.} and Blazer, {Vicki Suzette}",
year = "1997",
month = "1",
day = "1",
doi = "10.1016/S0380-1330(97)70890-6",
language = "English (US)",
volume = "23",
pages = "119--130",
journal = "Journal of Great Lakes Research",
issn = "0380-1330",
publisher = "International Association of Great Lakes Research",
number = "2",

}

Contaminant exposure, biochemical, and histopathological biomarkers in white suckers from contaminated and reference sites in the Sheboygan River, Wisconsin. / Schrank, Candy S.; Cormier, Susan M.; Blazer, Vicki Suzette.

In: Journal of Great Lakes Research, Vol. 23, No. 2, 01.01.1997, p. 119-130.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Contaminant exposure, biochemical, and histopathological biomarkers in white suckers from contaminated and reference sites in the Sheboygan River, Wisconsin

AU - Schrank, Candy S.

AU - Cormier, Susan M.

AU - Blazer, Vicki Suzette

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Fish populations of the lower Sheboygan River, located in east-central Wisconsin, are considered impaired under the Great Lakes Water Quality Agreement between the United States and Canada. Pollutants in the Sheboygan River system include: polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and heavy metals. This study compared general health and biochemical parameters, histology of selected organs, and contaminant residues and metabolites in a population of white suckers (Catostomus commersoni) from u contaminated reach of the Sheboygan River and an upstream reference site. Fish from the contaminated site had significantly lower hematocrits, significantly induced ethoxyresorufin-O-deethylase (EROD) activity as a measurement of hepatic mixed-function oxygenase activity, higher biliary metabolites of PAHs, and higher tissue concentrations of PCBs and p,p'-DDE relative to an upstream reference population. Analysis of covuriance suggests that both PCB and PAH exposure may be influencing EROD activities. Fish from the contaminated site featured more basophilic clusters and developing nephrons in kidney tissue suggesting the presence of a nephrotoxicant. Also, more fish exhibited hepatic lesions including diffuse cellular vacuolation, multifocal coagulative necrosis, bile ductal hyperplasia, and foci of cellular alteration which may be biomarkers for contaminant impacts. This study demonstrates that white suckers residing in the lower reaches of the Sheboygan River absorbed significant amounts of PAHs and PCBs and also exhibited hematological, biochemical and histological alterations some of which suggest impaired fish condition.

AB - Fish populations of the lower Sheboygan River, located in east-central Wisconsin, are considered impaired under the Great Lakes Water Quality Agreement between the United States and Canada. Pollutants in the Sheboygan River system include: polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), and heavy metals. This study compared general health and biochemical parameters, histology of selected organs, and contaminant residues and metabolites in a population of white suckers (Catostomus commersoni) from u contaminated reach of the Sheboygan River and an upstream reference site. Fish from the contaminated site had significantly lower hematocrits, significantly induced ethoxyresorufin-O-deethylase (EROD) activity as a measurement of hepatic mixed-function oxygenase activity, higher biliary metabolites of PAHs, and higher tissue concentrations of PCBs and p,p'-DDE relative to an upstream reference population. Analysis of covuriance suggests that both PCB and PAH exposure may be influencing EROD activities. Fish from the contaminated site featured more basophilic clusters and developing nephrons in kidney tissue suggesting the presence of a nephrotoxicant. Also, more fish exhibited hepatic lesions including diffuse cellular vacuolation, multifocal coagulative necrosis, bile ductal hyperplasia, and foci of cellular alteration which may be biomarkers for contaminant impacts. This study demonstrates that white suckers residing in the lower reaches of the Sheboygan River absorbed significant amounts of PAHs and PCBs and also exhibited hematological, biochemical and histological alterations some of which suggest impaired fish condition.

UR - http://www.scopus.com/inward/record.url?scp=0030874425&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030874425&partnerID=8YFLogxK

U2 - 10.1016/S0380-1330(97)70890-6

DO - 10.1016/S0380-1330(97)70890-6

M3 - Article

AN - SCOPUS:0030874425

VL - 23

SP - 119

EP - 130

JO - Journal of Great Lakes Research

JF - Journal of Great Lakes Research

SN - 0380-1330

IS - 2

ER -