Continuous Monitoring of Inflammation Biomarkers During Simulated Cardiopulmonary Bypass Using a Microfluidic Immunoassay Device-A Pilot Study

Lawrence A. Sasso, Kiana Aran, Yulong Guan, Akif Ündar, Jeffrey D. Zahn

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

This work demonstrates the use of a continuous online monitoring system for tracking systemic inflammation biomarkers during cardiopulmonary bypass (CPB) procedures. The ability to monitor inflammation biomarkers during CPB will allow surgical teams to actively treat inflammation and reduce harmful effects on postoperative morbidity and mortality, enabling improved patient outcomes. A microfluidic device has been designed which allows automation of the individual processing steps of a microbead immunoassay to allow continuous tracking of antigen concentrations. Preliminary experiments have demonstrated that the results produced by the microimmunoassay are comparable to results produced from a standard enzyme-linked immunosorbent assay (r=0.98). Additionally, integration of the assay with a simulated CPB circuit has been demonstrated with temporal tracking of C3a concentrations within blood continuously sampled from the circuit. The presented work describes the motivation, design challenges, and preliminary experimental results of this project.

Original languageEnglish (US)
Pages (from-to)E9-E17
JournalArtificial organs
Volume37
Issue number1
DOIs
StatePublished - Jan 1 2013

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Lab-On-A-Chip Devices
Biomarkers
Cardiopulmonary Bypass
Immunoassay
Microfluidics
Assays
Inflammation
Immunosorbents
Networks (circuits)
Monitoring
Antigens
Online Systems
Blood
Automation
Enzymes
Microspheres
Processing
Enzyme-Linked Immunosorbent Assay
Morbidity
Mortality

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

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Continuous Monitoring of Inflammation Biomarkers During Simulated Cardiopulmonary Bypass Using a Microfluidic Immunoassay Device-A Pilot Study. / Sasso, Lawrence A.; Aran, Kiana; Guan, Yulong; Ündar, Akif; Zahn, Jeffrey D.

In: Artificial organs, Vol. 37, No. 1, 01.01.2013, p. E9-E17.

Research output: Contribution to journalArticle

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