Continuous particle cross over in microfluidic channels for continuous biosensing

Sung Yang, Akif Undar, Jeffrey D. Zahn

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel concept for continuous biosensing is introduced. Continuous biosensing is achieved by the mechanism named "Particle Cross Over" within microfluidic channels. The functionality of the device is demonstrated by quantifying the binding events of biotin to streptavidin coated beads. The device is designed to have precisely controlled flow structures which allow cytometric beads to pass from one flow region to another without mixing the carrier and sample fluids. The mean and standard deviation of the cytometric bead pixel intensities are 43.52 and 19.39, respectively for 1 μg/ml of biotinylated Fluorescein IsoThioCyanate concentration with a binding time of 0.464 seconds and shows a Gaussian distribution. It is expected that the particle cross over mechanism introduced in this report will be very useful mechanism for applications in continuous biosensing.

Original languageEnglish (US)
Title of host publicationProceedings of 2006 ASME International Mechanical Engineering Congress and Exposition, IMECE2006 - Fluids Engineering Division
PublisherAmerican Society of Mechanical Engineers (ASME)
ISBN (Print)0791837904, 9780791837900
DOIs
StatePublished - Jan 1 2006
Event2006 ASME International Mechanical Engineering Congress and Exposition, IMECE2006 - Chicago, IL, United States
Duration: Nov 5 2006Nov 10 2006

Publication series

NameAmerican Society of Mechanical Engineers, Fluids Engineering Division (Publication) FED
ISSN (Print)0888-8116

Other

Other2006 ASME International Mechanical Engineering Congress and Exposition, IMECE2006
CountryUnited States
CityChicago, IL
Period11/5/0611/10/06

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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  • Cite this

    Yang, S., Undar, A., & Zahn, J. D. (2006). Continuous particle cross over in microfluidic channels for continuous biosensing. In Proceedings of 2006 ASME International Mechanical Engineering Congress and Exposition, IMECE2006 - Fluids Engineering Division (American Society of Mechanical Engineers, Fluids Engineering Division (Publication) FED). American Society of Mechanical Engineers (ASME). https://doi.org/10.1115/IMECE2006-16087