Contrasting mechanisms by which social isolation and restraint impair healing in male mice

Leah M. Pyter, Linglan Yang, Cassandra McKenzie, José M. Da Rocha, C. Sue Carter, Bin Cheng, Christopher Gerald Engeland

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Stress modulates vital aspects of immune functioning in both human and non-human animals, including tissue repair. For example, dermal wounds heal more slowly and are associated with prolonged inflammation and increased bacterial load in mice that experience chronic physical restraint. Social stressors also negatively affect healing; however, previous studies suggest that the affected healing mechanisms may be stress model-specific. Here, the effects of either social isolation or physical restraint on dermal wound healing (3.5mm wounds on the dorsum) were compared in hairless male mice. Social isolation beginning 3 weeks prior to wounding delayed healing comparably to physical restraint (12h/day for eight days), in spite of marked differences in metabolic and hormonal consequences (i.e. body mass) between the two stress models. Additionally, isolated mice exhibited reductions in wound bacterial load and inflammatory gene expression (interleukin-1beta [IL-1β], monocyte chemoattractant protein [MCP]), whereas restraint significantly increased both of these parameters relative to controls. Experimentally augmenting bacterial concentrations in wounds of isolated mice did not ameliorate healing, whereas this treatment accelerated healing in controls. This work indicates that social isolation and restraint stressors comparably impair healing, but do so through disparate mechanisms and at different phases of healing.

Original languageEnglish (US)
Pages (from-to)256-265
Number of pages10
JournalStress
Volume17
Issue number3
DOIs
StatePublished - Jan 1 2014

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Social Isolation
Physical Restraint
Bacterial Load
Wounds and Injuries
Monocyte Chemoattractant Proteins
Hairless Mouse
Skin
Interleukin-1beta
Wound Healing
Inflammation
Gene Expression
Therapeutics

All Science Journal Classification (ASJC) codes

  • Physiology
  • Neuropsychology and Physiological Psychology
  • Endocrine and Autonomic Systems
  • Psychiatry and Mental health
  • Behavioral Neuroscience

Cite this

Pyter, L. M., Yang, L., McKenzie, C., Da Rocha, J. M., Carter, C. S., Cheng, B., & Engeland, C. G. (2014). Contrasting mechanisms by which social isolation and restraint impair healing in male mice. Stress, 17(3), 256-265. https://doi.org/10.3109/10253890.2014.910761
Pyter, Leah M. ; Yang, Linglan ; McKenzie, Cassandra ; Da Rocha, José M. ; Carter, C. Sue ; Cheng, Bin ; Engeland, Christopher Gerald. / Contrasting mechanisms by which social isolation and restraint impair healing in male mice. In: Stress. 2014 ; Vol. 17, No. 3. pp. 256-265.
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Pyter, LM, Yang, L, McKenzie, C, Da Rocha, JM, Carter, CS, Cheng, B & Engeland, CG 2014, 'Contrasting mechanisms by which social isolation and restraint impair healing in male mice', Stress, vol. 17, no. 3, pp. 256-265. https://doi.org/10.3109/10253890.2014.910761

Contrasting mechanisms by which social isolation and restraint impair healing in male mice. / Pyter, Leah M.; Yang, Linglan; McKenzie, Cassandra; Da Rocha, José M.; Carter, C. Sue; Cheng, Bin; Engeland, Christopher Gerald.

In: Stress, Vol. 17, No. 3, 01.01.2014, p. 256-265.

Research output: Contribution to journalArticle

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Pyter LM, Yang L, McKenzie C, Da Rocha JM, Carter CS, Cheng B et al. Contrasting mechanisms by which social isolation and restraint impair healing in male mice. Stress. 2014 Jan 1;17(3):256-265. https://doi.org/10.3109/10253890.2014.910761