Contributions of contact activation pathways of coagulation factor XII in plasma

Kaushik Chatterjee, Zhe Guo, Erwin A. Vogler, Christopher A. Siedlecki

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25 Scopus citations

Abstract

Activation of human blood plasma coagulation by contact with hydrophilic or hydrophobic surfaces (procoagulants) is dominated by kallikrein (Kal)-mediated activation of the blood zymogen FXII (Hageman Factor). Mathematical modeling of prekallikrein (PK)-deficient platelet-poor plasma (dPKPPP) and PK-reconstituted dPKPPP (RdPKPPP) coagulation shows that autoactivation of FXII (FXII→surfaceFXIIa) produces no more than about 25% of the total FXIIa produced by the intrinsic pathway. Autoactivation and reciprocal-activation increase in the same proportion with procoagulant surface energy (water-wettability), whereas total amount of FXIIa produced per-unit-area procoagulant remains roughly constant for any particular procoagulant. These results suggest that procoagulant surfaces initiate the intrinsic cascade by producing a bolus of FXIIa in proportion to surface energy or surface area but play no additional role in subsequent molecular events in the cascade. Results further suggest that reciprocal-activation occurs in proportion to the amount of FXIIa produced by the initiating autoactivation step.

Original languageEnglish (US)
Pages (from-to)27-34
Number of pages8
JournalJournal of Biomedical Materials Research - Part A
Volume90
Issue number1
DOIs
StatePublished - Jul 1 2009

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All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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