Contributions of immune responses to developmental resistance in Lymantria dispar challenged with baculovirus

James McNeil, Diana Lynn Cox-foster, James Slavicek, Kelli Hoover

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

How the innate immune system functions to defend insects from viruses is an emerging field of study. We examined the impact of melanized encapsulation, a component of innate immunity that integrates both cellular and humoral immune responses, on the success of the baculovirus Lymantria dispar multiple nucleocapsid nucleopolyhedrovirus (LdMNPV) in its host L. dispar. L. dispar exhibits midgut-based and systemic, age-dependent resistance to LdMNPV within the fourth instar; the LD50 in newly molted larvae is approximately 18-fold lower than in mid-instar larvae (48-72h post-molt). We examined the role of the immune system in systemic resistance by measuring differences in hemocyte immunoresponsiveness to foreign targets, hemolymph phenoloxidase (PO) and FAD-glucose dehydrogenase (GLD) activities, and melanization of infected tissue culture cells. Mid-instar larvae showed a higher degree of hemocyte immunoresponsiveness, greater potential PO activity (pro-PO) at the time the virus is escaping the midgut to enter the hemocoel (72h post-inoculation), greater GLD activity, and more targeted melanization of infected tissue, which correlate with reduced viral success in the host. These findings support the hypothesis that innate immune responses can play an important role in anti-viral defenses against baculoviruses and that the success of these defenses can be age-dependent.

Original languageEnglish (US)
Pages (from-to)1167-1177
Number of pages11
JournalJournal of Insect Physiology
Volume56
Issue number9
DOIs
StatePublished - Sep 1 2010

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Lymantria dispar
Baculoviridae
Glucose 1-Dehydrogenase
Nucleopolyhedrovirus
Larva
Hemocytes
Nucleocapsid
Monophenol Monooxygenase
immune response
Innate Immunity
nucleocapsid
melanization
instars
Immune System
monophenol monooxygenase
Insect Viruses
hemocytes
midgut
larvae
Flavin-Adenine Dinucleotide

All Science Journal Classification (ASJC) codes

  • Physiology
  • Insect Science

Cite this

McNeil, James ; Cox-foster, Diana Lynn ; Slavicek, James ; Hoover, Kelli. / Contributions of immune responses to developmental resistance in Lymantria dispar challenged with baculovirus. In: Journal of Insect Physiology. 2010 ; Vol. 56, No. 9. pp. 1167-1177.
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Contributions of immune responses to developmental resistance in Lymantria dispar challenged with baculovirus. / McNeil, James; Cox-foster, Diana Lynn; Slavicek, James; Hoover, Kelli.

In: Journal of Insect Physiology, Vol. 56, No. 9, 01.09.2010, p. 1167-1177.

Research output: Contribution to journalArticle

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