Control of circulating IgE by the Vitamin D receptor in vivo involves B cell intrinsic and extrinsic mechanisms

Jamaal James, Veronika Weaver, Margherita T. Cantorna

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Vitamin D deficiency is associated with the development of asthma and allergy. The active form of vitamin D [1,25(OH)2D] regulates B cells in vitro and mice without the vitamin D receptor (VDR knockout [KO]) have high serum IgE. Whole-body VDR KO, T cell-specific VDR (T-VDR) KO, B cell-specific VDR (B-VDR) KO, and vitamin D deficient mice were used to determine the targets of vitamin D in the regulation of IgE in vivo. Vitamin D deficient, VDR KO, and B-VDR KO mice developed hyper-IgE, whereas T-VDR KO mice did not. The data show that IL-10 secretion by B cells and CD1d expression on IL-10 secreting B cells was lower in VDR KO mice. Mesenteric lymph node cultures from VDR KO and B-VDR KO mice secreted higher IgE ex vivo than wild-type (WT) cultures, and the addition of IL-10 eliminated the difference in IgE production between VDR KO and WT cultures. The increase in IgE in VDR KO mice was 2-fold greater than in the B-VDR KO mice, suggesting that VDR deficiency in non-B cells contributes to hyper-IgE in vivo. Antibiotic depletion of the microbiota raised serum IgE 4-fold in both WTand VDR KO mice. The VDR directly and indirectly regulates IgE production in B cells. Through the VDR, vitamin D is an environmental factor that helps to maintain low serum IgE responses.

Original languageEnglish (US)
Pages (from-to)1164-1171
Number of pages8
JournalJournal of Immunology
Volume198
Issue number3
DOIs
StatePublished - Feb 1 2017

Fingerprint

Calcitriol Receptors
Immunoglobulin E
B-Lymphocytes
Knockout Mice
Vitamin D
Interleukin-10
Serum
T-Lymphocytes
Vitamin D Deficiency
Microbiota
Hypersensitivity
Asthma
Lymph Nodes
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

@article{cb6fdeb6759545c59e69a390617747eb,
title = "Control of circulating IgE by the Vitamin D receptor in vivo involves B cell intrinsic and extrinsic mechanisms",
abstract = "Vitamin D deficiency is associated with the development of asthma and allergy. The active form of vitamin D [1,25(OH)2D] regulates B cells in vitro and mice without the vitamin D receptor (VDR knockout [KO]) have high serum IgE. Whole-body VDR KO, T cell-specific VDR (T-VDR) KO, B cell-specific VDR (B-VDR) KO, and vitamin D deficient mice were used to determine the targets of vitamin D in the regulation of IgE in vivo. Vitamin D deficient, VDR KO, and B-VDR KO mice developed hyper-IgE, whereas T-VDR KO mice did not. The data show that IL-10 secretion by B cells and CD1d expression on IL-10 secreting B cells was lower in VDR KO mice. Mesenteric lymph node cultures from VDR KO and B-VDR KO mice secreted higher IgE ex vivo than wild-type (WT) cultures, and the addition of IL-10 eliminated the difference in IgE production between VDR KO and WT cultures. The increase in IgE in VDR KO mice was 2-fold greater than in the B-VDR KO mice, suggesting that VDR deficiency in non-B cells contributes to hyper-IgE in vivo. Antibiotic depletion of the microbiota raised serum IgE 4-fold in both WTand VDR KO mice. The VDR directly and indirectly regulates IgE production in B cells. Through the VDR, vitamin D is an environmental factor that helps to maintain low serum IgE responses.",
author = "Jamaal James and Veronika Weaver and Cantorna, {Margherita T.}",
year = "2017",
month = "2",
day = "1",
doi = "10.4049/jimmunol.16012133",
language = "English (US)",
volume = "198",
pages = "1164--1171",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

Control of circulating IgE by the Vitamin D receptor in vivo involves B cell intrinsic and extrinsic mechanisms. / James, Jamaal; Weaver, Veronika; Cantorna, Margherita T.

In: Journal of Immunology, Vol. 198, No. 3, 01.02.2017, p. 1164-1171.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Control of circulating IgE by the Vitamin D receptor in vivo involves B cell intrinsic and extrinsic mechanisms

AU - James, Jamaal

AU - Weaver, Veronika

AU - Cantorna, Margherita T.

PY - 2017/2/1

Y1 - 2017/2/1

N2 - Vitamin D deficiency is associated with the development of asthma and allergy. The active form of vitamin D [1,25(OH)2D] regulates B cells in vitro and mice without the vitamin D receptor (VDR knockout [KO]) have high serum IgE. Whole-body VDR KO, T cell-specific VDR (T-VDR) KO, B cell-specific VDR (B-VDR) KO, and vitamin D deficient mice were used to determine the targets of vitamin D in the regulation of IgE in vivo. Vitamin D deficient, VDR KO, and B-VDR KO mice developed hyper-IgE, whereas T-VDR KO mice did not. The data show that IL-10 secretion by B cells and CD1d expression on IL-10 secreting B cells was lower in VDR KO mice. Mesenteric lymph node cultures from VDR KO and B-VDR KO mice secreted higher IgE ex vivo than wild-type (WT) cultures, and the addition of IL-10 eliminated the difference in IgE production between VDR KO and WT cultures. The increase in IgE in VDR KO mice was 2-fold greater than in the B-VDR KO mice, suggesting that VDR deficiency in non-B cells contributes to hyper-IgE in vivo. Antibiotic depletion of the microbiota raised serum IgE 4-fold in both WTand VDR KO mice. The VDR directly and indirectly regulates IgE production in B cells. Through the VDR, vitamin D is an environmental factor that helps to maintain low serum IgE responses.

AB - Vitamin D deficiency is associated with the development of asthma and allergy. The active form of vitamin D [1,25(OH)2D] regulates B cells in vitro and mice without the vitamin D receptor (VDR knockout [KO]) have high serum IgE. Whole-body VDR KO, T cell-specific VDR (T-VDR) KO, B cell-specific VDR (B-VDR) KO, and vitamin D deficient mice were used to determine the targets of vitamin D in the regulation of IgE in vivo. Vitamin D deficient, VDR KO, and B-VDR KO mice developed hyper-IgE, whereas T-VDR KO mice did not. The data show that IL-10 secretion by B cells and CD1d expression on IL-10 secreting B cells was lower in VDR KO mice. Mesenteric lymph node cultures from VDR KO and B-VDR KO mice secreted higher IgE ex vivo than wild-type (WT) cultures, and the addition of IL-10 eliminated the difference in IgE production between VDR KO and WT cultures. The increase in IgE in VDR KO mice was 2-fold greater than in the B-VDR KO mice, suggesting that VDR deficiency in non-B cells contributes to hyper-IgE in vivo. Antibiotic depletion of the microbiota raised serum IgE 4-fold in both WTand VDR KO mice. The VDR directly and indirectly regulates IgE production in B cells. Through the VDR, vitamin D is an environmental factor that helps to maintain low serum IgE responses.

UR - http://www.scopus.com/inward/record.url?scp=85014677067&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014677067&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.16012133

DO - 10.4049/jimmunol.16012133

M3 - Article

C2 - 28003380

AN - SCOPUS:85014677067

VL - 198

SP - 1164

EP - 1171

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -