Control of rice grain-filling and yield by a gene with a potential signature of domestication

Ertao Wang, Jianjun Wang, Xudong Zhu, Wei Hao, Linyou Wang, Qun Li, Lixia Zhang, Wei He, Baorong Lu, Hongxuan Lin, Hong Ma, Guiquan Zhang, Zuhua He

Research output: Contribution to journalArticle

384 Citations (Scopus)

Abstract

Grain-filling, an important trait that contributes greatly to grain weight, is regulated by quantitative trait loci and is associated with crop domestication syndrome. However, the genes and underlying molecular mechanisms controlling crop grain-filling remain elusive. Here we report the isolation and functional analysis of the rice GIF1 (GRAIN INCOMPLETE FILLING 1) gene that encodes a cell-wall invertase required for carbon partitioning during early grain-filling. The cultivated GIF1 gene shows a restricted expression pattern during grain-filling compared to the wild rice allele, probably a result of accumulated mutations in the gene's regulatory sequence through domestication. Fine mapping with introgression lines revealed that the wild rice GIF1 is responsible for grain weight reduction. Ectopic expression of the cultivated GIF1 gene with the 35S or rice Waxy promoter resulted in smaller grains, whereas overexpression of GIF1 driven by its native promoter increased grain production. These findings, together with the domestication signature that we identified by comparing nucleotide diversity of the GIF1 loci between cultivated and wild rice, strongly suggest that GIF1 is a potential domestication gene and that such a domestication-selected gene can be used for further crop improvement.

Original languageEnglish (US)
Pages (from-to)1370-1374
Number of pages5
JournalNature Genetics
Volume40
Issue number11
DOIs
StatePublished - Nov 1 2008

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Genes
beta-Fructofuranosidase
Quantitative Trait Loci
Regulator Genes
Cell Wall
Oryza
Domestication
Weight Loss
Carbon
Nucleotides
Alleles
Weights and Measures
Mutation
Ectopic Gene Expression

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Wang, E., Wang, J., Zhu, X., Hao, W., Wang, L., Li, Q., ... He, Z. (2008). Control of rice grain-filling and yield by a gene with a potential signature of domestication. Nature Genetics, 40(11), 1370-1374. https://doi.org/10.1038/ng.220
Wang, Ertao ; Wang, Jianjun ; Zhu, Xudong ; Hao, Wei ; Wang, Linyou ; Li, Qun ; Zhang, Lixia ; He, Wei ; Lu, Baorong ; Lin, Hongxuan ; Ma, Hong ; Zhang, Guiquan ; He, Zuhua. / Control of rice grain-filling and yield by a gene with a potential signature of domestication. In: Nature Genetics. 2008 ; Vol. 40, No. 11. pp. 1370-1374.
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Wang, E, Wang, J, Zhu, X, Hao, W, Wang, L, Li, Q, Zhang, L, He, W, Lu, B, Lin, H, Ma, H, Zhang, G & He, Z 2008, 'Control of rice grain-filling and yield by a gene with a potential signature of domestication', Nature Genetics, vol. 40, no. 11, pp. 1370-1374. https://doi.org/10.1038/ng.220

Control of rice grain-filling and yield by a gene with a potential signature of domestication. / Wang, Ertao; Wang, Jianjun; Zhu, Xudong; Hao, Wei; Wang, Linyou; Li, Qun; Zhang, Lixia; He, Wei; Lu, Baorong; Lin, Hongxuan; Ma, Hong; Zhang, Guiquan; He, Zuhua.

In: Nature Genetics, Vol. 40, No. 11, 01.11.2008, p. 1370-1374.

Research output: Contribution to journalArticle

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