Control of the papillomavirus early-to-late switch by differentially expressed SRp20

Rong Jia, Xuefeng Liu, Mingfang Tao, Michael Kruhlak, Ming Guo, Craig Meyers, Carl C. Baker, Zhi Ming Zheng

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

The viral early-to-late switch of papillomavirus infection is tightly linked to keratinocyte differentiation and is mediated in part by alternative mRNA splicing. Here, we report that SRp20, a cellular splicing factor, controls the early-to-late switch via interactions with A/C-rich RNA elements. An A/C-rich SE4 element regulates the selection of a bovine papillomavirus type 1 (BPV-1) late-specific splice site, and binding of SRp20 to SE4 suppresses this selection. Expression of late BPV-1 L1 or human papillomavirus (HPV) L1, the major capsid protein, inversely correlates with SRp20 levels in the terminally differentiated keratinocytes. In HPV type 16, a similar SRp20-interacting element also controls the viral early-to-late switch. Keratinocytes in raft cultures, which support L1 expression, make considerably less SRp20 than keratinocytes in monolayer cultures, which do not support L1 expression. Conversely, abundant SRp20 in cancer cells or undifferentiated keratinocytes is important for the expression of the viral early E6 and E7 by promoting the expression of cellular transcription factor SP1 for transactivation of viral early promoters.

Original languageEnglish (US)
Pages (from-to)167-180
Number of pages14
JournalJournal of virology
Volume83
Issue number1
DOIs
StatePublished - Jan 1 2009

Fingerprint

Papillomaviridae
keratinocytes
Keratinocytes
Bovine papillomavirus 1
Papillomavirus Infections
Human papillomavirus 16
Alternative Splicing
Capsid Proteins
transcriptional activation
coat proteins
Transcriptional Activation
Transcription Factors
transcription factors
Binding Sites
promoter regions
RNA
Messenger RNA
infection
Neoplasms

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Jia, Rong ; Liu, Xuefeng ; Tao, Mingfang ; Kruhlak, Michael ; Guo, Ming ; Meyers, Craig ; Baker, Carl C. ; Zheng, Zhi Ming. / Control of the papillomavirus early-to-late switch by differentially expressed SRp20. In: Journal of virology. 2009 ; Vol. 83, No. 1. pp. 167-180.
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Jia, R, Liu, X, Tao, M, Kruhlak, M, Guo, M, Meyers, C, Baker, CC & Zheng, ZM 2009, 'Control of the papillomavirus early-to-late switch by differentially expressed SRp20', Journal of virology, vol. 83, no. 1, pp. 167-180. https://doi.org/10.1128/JVI.01719-08

Control of the papillomavirus early-to-late switch by differentially expressed SRp20. / Jia, Rong; Liu, Xuefeng; Tao, Mingfang; Kruhlak, Michael; Guo, Ming; Meyers, Craig; Baker, Carl C.; Zheng, Zhi Ming.

In: Journal of virology, Vol. 83, No. 1, 01.01.2009, p. 167-180.

Research output: Contribution to journalArticle

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