Local composition, structure, morphology, and phase are interrelated in lipid bilayer membranes. This gives us the opportunity to control one or more of these properties by manipulating others. We investigate these relationships with combinations of simultaneous two-color widefield fluorescence imaging, three-dimensional rendering of vesicle domains, and manipulation of the vesicle morphology via optical trapping and micropipette aspiration. We describe methods to modulate, to measure, and to probe the local structure of model membranes through control of membrane curvature in liposomes.
All Science Journal Classification (ASJC) codes
- Atomic and Molecular Physics, and Optics
- Molecular Biology
- Cell Biology