Controlling and measuring local composition and properties in lipid bilayer membranes

T. G. D’Onofrio, C. W. Binns, E. H. Muth, C. D. Keating, P. S. Weiss

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Local composition, structure, morphology, and phase are interrelated in lipid bilayer membranes. This gives us the opportunity to control one or more of these properties by manipulating others. We investigate these relationships with combinations of simultaneous two-color widefield fluorescence imaging, three-dimensional rendering of vesicle domains, and manipulation of the vesicle morphology via optical trapping and micropipette aspiration. We describe methods to modulate, to measure, and to probe the local structure of model membranes through control of membrane curvature in liposomes.

Original languageEnglish (US)
Article number399163
Pages (from-to)605-617
Number of pages13
JournalJournal of Biological Physics
Volume28
Issue number4
DOIs
StatePublished - Jan 1 2002

Fingerprint

Lipid Bilayers
lipids
membranes
Membranes
Optical Tweezers
Optical Imaging
Liposomes
manipulators
Color
trapping
curvature
color
fluorescence
vacuum
probes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Atomic and Molecular Physics, and Optics
  • Molecular Biology
  • Cell Biology

Cite this

D’Onofrio, T. G. ; Binns, C. W. ; Muth, E. H. ; Keating, C. D. ; Weiss, P. S. / Controlling and measuring local composition and properties in lipid bilayer membranes. In: Journal of Biological Physics. 2002 ; Vol. 28, No. 4. pp. 605-617.
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Controlling and measuring local composition and properties in lipid bilayer membranes. / D’Onofrio, T. G.; Binns, C. W.; Muth, E. H.; Keating, C. D.; Weiss, P. S.

In: Journal of Biological Physics, Vol. 28, No. 4, 399163, 01.01.2002, p. 605-617.

Research output: Contribution to journalArticle

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