Converging pathways lead to overproduction of IL-17 in the absence of vitamin D signaling

Danny Bruce, Sanhong Yu, Jot Hui Ooi, Margherita T. Cantorna

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Multiple pathways converge to result in the overexpression of T h17 cells in the absence of either vitamin D or the vitamin D receptor (VDR). CD4 + T cells from VDR knockout (KO) mice have a more activated phenotype than their wild-type (WT) counterparts and readily develop into T h17 cells under a variety of in vitro conditions. Vitamin D-deficient CD4 + T cells also overproduced IL-17 in vitro and 1,25 dihydroxyvitamin D 3 inhibited the development of T h17 cells in CD4 + T-cell cultures. Conversely, the induction of inducible (i) Tregs was lower in VDR KO CD4 + T cells than WT and the VDR KO iTregs were refractory to IL-6 inhibition. Host-specific effects of the VDR were evident on in vivo development of naive T cells. Development of naive WT CD4 + T cells in the VDR KO host resulted in the overexpression of IL-17 and more severe experimental inflammatory bowel disease (IBD). The increased expression of T h17 cells in the VDR KO mice was associated with a reduction in tolerogenic CD103 + dendritic cells. The data collectively demonstrate that T h17 and iTreg cells are direct and indirect targets of vitamin D. The increased propensity for development of T h17 cells in the VDR KO host results in more severe IBD.

Original languageEnglish (US)
Pages (from-to)519-528
Number of pages10
JournalInternational immunology
Volume23
Issue number8
DOIs
StatePublished - 2011

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Converging pathways lead to overproduction of IL-17 in the absence of vitamin D signaling'. Together they form a unique fingerprint.

  • Cite this