Cloned human lamin C was expressed in and purified from bacteria and used in ATP binding assays. Scatchard analysis revealed strong positive cooperative and noncooperative binding, with estimated apparent dissociation constants of 3 × 10-6 and 2 × 10-5 M, respectively. The binding is strongly pH dependent. ATP binding by lamins A C (presumably as intermediate filaments) may provide a substantial storage depot for ATP at the peripheral lamina for use by a number of ATP-requiring nuclear scaffold enzymes.
All Science Journal Classification (ASJC) codes
- Cell Biology