Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A

K. Sivakumaran; M. H. Chen; M. J. Roossinck; C. C. Kao

doi:10.1046/j.1464-6722.2001.00089.x

Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A

K. Sivakumaran, M. H. Chen, M. J. Roossinck, C. C. Kao

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18 Citations (SciVal)

Abstract

The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3′ of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3′ of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.

Original language	English (US)
Pages (from-to)	43-52
Number of pages	10
Journal	Molecular plant pathology
Volume	3
Issue number	1
DOIs	https://doi.org/10.1046/j.1464-6722.2001.00089.x
State	Published - Mar 4 2002

All Science Journal Classification (ASJC) codes

Molecular Biology
Agronomy and Crop Science
Soil Science
Plant Science

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10.1046/j.1464-6722.2001.00089.x

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abstract = "The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3′ of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3′ of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.",

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AU - Chen, M. H.

AU - Roossinck, M. J.

AU - Kao, C. C.

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Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A

Abstract

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