The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3′ of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3′ of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Agronomy and Crop Science
- Soil Science
- Plant Science