Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A

K. Sivakumaran, M. H. Chen, Marilyn J. Roossinck, C. C. Kao

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3′ of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3′ of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.

Original languageEnglish (US)
Pages (from-to)43-52
Number of pages10
JournalMolecular Plant Pathology
Volume3
Issue number1
DOIs
StatePublished - 2002

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Cucumovirus
Cucumber mosaic virus
RNA
virus
promoter regions
Nucleotides
nucleotides
synthesis
secondary structure
stem
assay
nucleotide sequences
stems
assays
mosaic
3-monoiodothyronine

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Sivakumaran, K. ; Chen, M. H. ; Roossinck, Marilyn J. ; Kao, C. C. / Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A. In: Molecular Plant Pathology. 2002 ; Vol. 3, No. 1. pp. 43-52.
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Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A. / Sivakumaran, K.; Chen, M. H.; Roossinck, Marilyn J.; Kao, C. C.

In: Molecular Plant Pathology, Vol. 3, No. 1, 2002, p. 43-52.

Research output: Contribution to journalArticle

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AU - Chen, M. H.

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