Core promoter for initiation of Cucumber mosaic virus subgenomic RNA4A

K. Sivakumaran, M. H. Chen, M. J. Roossinck, C. C. Kao

Research output: Contribution to journalArticlepeer-review

18 Citations (SciVal)


The core subgenomic promoter for the initiation of Cucumber mosaic virus (CMV) RNA4A was characterized in vitro using a template-dependent RNA synthesis assay and variants of the core promoter RNAs. The minimal sequence required for specific initiation from the cytidylate (T1) used in vivo consists of 31-nucleotides (nt) 3′ of T1 and a 13 nt template sequence. This 44 nt RNA was found to provide three elements that contribute to efficient initiation of RNA4A synthesis by the CMV replicase: a stem-loop secondary structure 3′ of T1, a template sequence that is rich in adenylates and uridylates, and T1 in an unbase-paired sequence.

Original languageEnglish (US)
Pages (from-to)43-52
Number of pages10
JournalMolecular plant pathology
Issue number1
StatePublished - Mar 4 2002

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Agronomy and Crop Science
  • Soil Science
  • Plant Science


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