Correlation between crystalline cellulose structure and cellulose synthase complex shape: a spectroscopic study with unicellular freshwater alga Micrasterias

Shixin Huang, Sarah N. Kiemle, Mohamadamin Makarem, Seong H. Kim

Research output: Contribution to journalArticle

Abstract

Cellulose is synthesized into fibrils by enzyme complexes called cellulose synthase complexes (CSCs). We analyzed cellulose fibrils produced in a dividing unicellular freshwater alga Micrasterias with vibrational sum frequency generation (SFG) spectroscopy. It is known that cellulose in the daughter semi-cell of Micrasterias is synthesized by the isolated individual rosette-shape CSC, while cellulose in the mother semi-cell is produced by hexagonal arrays of rosette-shape CSCs. Comparing the SFG spectra of cellulose in these two regions of a single cell of Micrasterias showed intriguing correlations between the polymorphic structure and crystal size of cellulose and the shape and size of the CSCs. This information may provide a foundation needed to understand what determines the cellulose structure and crystallinity in plant cell walls.

Original languageEnglish (US)
Pages (from-to)57-69
Number of pages13
JournalCellulose
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2020

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Algae
Cellulose
Crystalline materials
Vibrational spectra
cellulose synthase
Spectroscopy
Crystals
Enzymes

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics

Cite this

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abstract = "Cellulose is synthesized into fibrils by enzyme complexes called cellulose synthase complexes (CSCs). We analyzed cellulose fibrils produced in a dividing unicellular freshwater alga Micrasterias with vibrational sum frequency generation (SFG) spectroscopy. It is known that cellulose in the daughter semi-cell of Micrasterias is synthesized by the isolated individual rosette-shape CSC, while cellulose in the mother semi-cell is produced by hexagonal arrays of rosette-shape CSCs. Comparing the SFG spectra of cellulose in these two regions of a single cell of Micrasterias showed intriguing correlations between the polymorphic structure and crystal size of cellulose and the shape and size of the CSCs. This information may provide a foundation needed to understand what determines the cellulose structure and crystallinity in plant cell walls.",
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Correlation between crystalline cellulose structure and cellulose synthase complex shape : a spectroscopic study with unicellular freshwater alga Micrasterias. / Huang, Shixin; Kiemle, Sarah N.; Makarem, Mohamadamin; Kim, Seong H.

In: Cellulose, Vol. 27, No. 1, 01.01.2020, p. 57-69.

Research output: Contribution to journalArticle

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AB - Cellulose is synthesized into fibrils by enzyme complexes called cellulose synthase complexes (CSCs). We analyzed cellulose fibrils produced in a dividing unicellular freshwater alga Micrasterias with vibrational sum frequency generation (SFG) spectroscopy. It is known that cellulose in the daughter semi-cell of Micrasterias is synthesized by the isolated individual rosette-shape CSC, while cellulose in the mother semi-cell is produced by hexagonal arrays of rosette-shape CSCs. Comparing the SFG spectra of cellulose in these two regions of a single cell of Micrasterias showed intriguing correlations between the polymorphic structure and crystal size of cellulose and the shape and size of the CSCs. This information may provide a foundation needed to understand what determines the cellulose structure and crystallinity in plant cell walls.

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