Correlation of CD2 expression with PML gene breakpoints in patients with acute promyelocytic leukemia

David Claxton, C. L. Reading, L. Nagarajan, Y. Tsujimoto, B. S. Andersson, E. Estey, A. Cork, Y. O. Huh, J. Trujillo, A. B. Deisseroth

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The chromosomal translocation t(15;17)(q22:21) of acute promyelocytic leukemia (APL) fuses PML, a novel gene, with RARα, a retinoic acid receptor gene. PML-RAR hybrid transcripts were studied in 18 cases of APL using RNA- PCR. Two forms were noted: one designated 5', producing a 439-bp chimeric fragment, and a 3' form, producing a pair of fragments of 765 bp and 909 bp. 5' forms were found in 7 of the 18 cases while the other 11 patients expressed the 3' forms. The chromosome 15 specific probes K3 and K2 were used to study genomic breakpoints in 12 APL patients. Comparison of these results with RNA PCR in 11 patients for whom both were available yielded a rearrangement pattern predictive of whether the hybrid transcript was 5' or 3'. In this way, an additional three patients in whom DNA but not RNA was available were identified as having 3' (downstream) breakpoints and, therefore, 3' hybrid forms. Thus, 21 cases categorized as having 5' or 3' PML-RAR transcripts were analyzed for various phenotypic differences. Surface phenotyping of leukemic promyelocytes demonstrated expression of the CD2 antigen in all cases with the 5' splice variant. Only 1 of 11 cases with the 3' form showed CD2 expression. This difference is significant at P = .001.

Original languageEnglish (US)
Pages (from-to)582-586
Number of pages5
JournalBlood
Volume80
Issue number3
StatePublished - Jan 1 1992

Fingerprint

Acute Promyelocytic Leukemia
Genes
RNA
CD2 Antigens
Retinoic Acid Receptors
Electric fuses
Chromosomes
Chromosomes, Human, Pair 15
Granulocyte Precursor Cells
Polymerase Chain Reaction
Genetic Translocation
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Claxton, D., Reading, C. L., Nagarajan, L., Tsujimoto, Y., Andersson, B. S., Estey, E., ... Deisseroth, A. B. (1992). Correlation of CD2 expression with PML gene breakpoints in patients with acute promyelocytic leukemia. Blood, 80(3), 582-586.
Claxton, David ; Reading, C. L. ; Nagarajan, L. ; Tsujimoto, Y. ; Andersson, B. S. ; Estey, E. ; Cork, A. ; Huh, Y. O. ; Trujillo, J. ; Deisseroth, A. B. / Correlation of CD2 expression with PML gene breakpoints in patients with acute promyelocytic leukemia. In: Blood. 1992 ; Vol. 80, No. 3. pp. 582-586.
@article{a02c7486e0fb4c6faff18df970ab2e2c,
title = "Correlation of CD2 expression with PML gene breakpoints in patients with acute promyelocytic leukemia",
abstract = "The chromosomal translocation t(15;17)(q22:21) of acute promyelocytic leukemia (APL) fuses PML, a novel gene, with RARα, a retinoic acid receptor gene. PML-RAR hybrid transcripts were studied in 18 cases of APL using RNA- PCR. Two forms were noted: one designated 5', producing a 439-bp chimeric fragment, and a 3' form, producing a pair of fragments of 765 bp and 909 bp. 5' forms were found in 7 of the 18 cases while the other 11 patients expressed the 3' forms. The chromosome 15 specific probes K3 and K2 were used to study genomic breakpoints in 12 APL patients. Comparison of these results with RNA PCR in 11 patients for whom both were available yielded a rearrangement pattern predictive of whether the hybrid transcript was 5' or 3'. In this way, an additional three patients in whom DNA but not RNA was available were identified as having 3' (downstream) breakpoints and, therefore, 3' hybrid forms. Thus, 21 cases categorized as having 5' or 3' PML-RAR transcripts were analyzed for various phenotypic differences. Surface phenotyping of leukemic promyelocytes demonstrated expression of the CD2 antigen in all cases with the 5' splice variant. Only 1 of 11 cases with the 3' form showed CD2 expression. This difference is significant at P = .001.",
author = "David Claxton and Reading, {C. L.} and L. Nagarajan and Y. Tsujimoto and Andersson, {B. S.} and E. Estey and A. Cork and Huh, {Y. O.} and J. Trujillo and Deisseroth, {A. B.}",
year = "1992",
month = "1",
day = "1",
language = "English (US)",
volume = "80",
pages = "582--586",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "3",

}

Claxton, D, Reading, CL, Nagarajan, L, Tsujimoto, Y, Andersson, BS, Estey, E, Cork, A, Huh, YO, Trujillo, J & Deisseroth, AB 1992, 'Correlation of CD2 expression with PML gene breakpoints in patients with acute promyelocytic leukemia', Blood, vol. 80, no. 3, pp. 582-586.

Correlation of CD2 expression with PML gene breakpoints in patients with acute promyelocytic leukemia. / Claxton, David; Reading, C. L.; Nagarajan, L.; Tsujimoto, Y.; Andersson, B. S.; Estey, E.; Cork, A.; Huh, Y. O.; Trujillo, J.; Deisseroth, A. B.

In: Blood, Vol. 80, No. 3, 01.01.1992, p. 582-586.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Correlation of CD2 expression with PML gene breakpoints in patients with acute promyelocytic leukemia

AU - Claxton, David

AU - Reading, C. L.

AU - Nagarajan, L.

AU - Tsujimoto, Y.

AU - Andersson, B. S.

AU - Estey, E.

AU - Cork, A.

AU - Huh, Y. O.

AU - Trujillo, J.

AU - Deisseroth, A. B.

PY - 1992/1/1

Y1 - 1992/1/1

N2 - The chromosomal translocation t(15;17)(q22:21) of acute promyelocytic leukemia (APL) fuses PML, a novel gene, with RARα, a retinoic acid receptor gene. PML-RAR hybrid transcripts were studied in 18 cases of APL using RNA- PCR. Two forms were noted: one designated 5', producing a 439-bp chimeric fragment, and a 3' form, producing a pair of fragments of 765 bp and 909 bp. 5' forms were found in 7 of the 18 cases while the other 11 patients expressed the 3' forms. The chromosome 15 specific probes K3 and K2 were used to study genomic breakpoints in 12 APL patients. Comparison of these results with RNA PCR in 11 patients for whom both were available yielded a rearrangement pattern predictive of whether the hybrid transcript was 5' or 3'. In this way, an additional three patients in whom DNA but not RNA was available were identified as having 3' (downstream) breakpoints and, therefore, 3' hybrid forms. Thus, 21 cases categorized as having 5' or 3' PML-RAR transcripts were analyzed for various phenotypic differences. Surface phenotyping of leukemic promyelocytes demonstrated expression of the CD2 antigen in all cases with the 5' splice variant. Only 1 of 11 cases with the 3' form showed CD2 expression. This difference is significant at P = .001.

AB - The chromosomal translocation t(15;17)(q22:21) of acute promyelocytic leukemia (APL) fuses PML, a novel gene, with RARα, a retinoic acid receptor gene. PML-RAR hybrid transcripts were studied in 18 cases of APL using RNA- PCR. Two forms were noted: one designated 5', producing a 439-bp chimeric fragment, and a 3' form, producing a pair of fragments of 765 bp and 909 bp. 5' forms were found in 7 of the 18 cases while the other 11 patients expressed the 3' forms. The chromosome 15 specific probes K3 and K2 were used to study genomic breakpoints in 12 APL patients. Comparison of these results with RNA PCR in 11 patients for whom both were available yielded a rearrangement pattern predictive of whether the hybrid transcript was 5' or 3'. In this way, an additional three patients in whom DNA but not RNA was available were identified as having 3' (downstream) breakpoints and, therefore, 3' hybrid forms. Thus, 21 cases categorized as having 5' or 3' PML-RAR transcripts were analyzed for various phenotypic differences. Surface phenotyping of leukemic promyelocytes demonstrated expression of the CD2 antigen in all cases with the 5' splice variant. Only 1 of 11 cases with the 3' form showed CD2 expression. This difference is significant at P = .001.

UR - http://www.scopus.com/inward/record.url?scp=0026691334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026691334&partnerID=8YFLogxK

M3 - Article

VL - 80

SP - 582

EP - 586

JO - Blood

JF - Blood

SN - 0006-4971

IS - 3

ER -

Claxton D, Reading CL, Nagarajan L, Tsujimoto Y, Andersson BS, Estey E et al. Correlation of CD2 expression with PML gene breakpoints in patients with acute promyelocytic leukemia. Blood. 1992 Jan 1;80(3):582-586.