Correlation of cleavage of SNAP-25 with muscle function in a rat model of Botulinum neurotoxin type A induced paralysis

Christine V. Jurasinski, Erich Lieth, An N. Dang Do, Cara-Lynne Schengrund

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Injection of botulinum neurotoxin serotype A (BoNT/A) into muscle results in cleavage of the synaptosomal associated protein of 25 kDa (SNAP-25) and relatively long-term paralysis. However, nerve-terminal sprouting, which appears to require intact SNAP-25, has been reported to occur much earlier. The difference between the long-term paralysis induced by injection of BoNT/A and the short time needed for sprouting led us to investigate the relationship between BoNT/A catalyzed cleavage of SNAP-25 and muscle function. The effect of BoNT/A on SNAP-25 present in nerve endings innervating gastrocnemius muscles of rats was monitored over time. Cleaved SNAP-25 was found in nerve terminals innervating the muscles within 24 h of inoculation with BoNT/A and was present more than 2 months later. Comparison of the ratios of cleaved to intact SNAP-25 from the onset of BoNT/A-induced paralysis until function was regained indicated that paralysis was probable when the ratio of cleaved to intact SNAP-25 was greater than 0.35.

Original languageEnglish (US)
Pages (from-to)1309-1315
Number of pages7
JournalToxicon
Volume39
Issue number9
DOIs
StatePublished - Jun 18 2001

Fingerprint

Synaptosomal-Associated Protein 25
Type A Botulinum Toxins
Paralysis
Neurotoxins
Muscle
Rats
Muscles
Injections
Nerve Endings
Serogroup
Skeletal Muscle

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Jurasinski, Christine V. ; Lieth, Erich ; Dang Do, An N. ; Schengrund, Cara-Lynne. / Correlation of cleavage of SNAP-25 with muscle function in a rat model of Botulinum neurotoxin type A induced paralysis. In: Toxicon. 2001 ; Vol. 39, No. 9. pp. 1309-1315.
@article{e813a913bff54eb3823ebd1c71a057ec,
title = "Correlation of cleavage of SNAP-25 with muscle function in a rat model of Botulinum neurotoxin type A induced paralysis",
abstract = "Injection of botulinum neurotoxin serotype A (BoNT/A) into muscle results in cleavage of the synaptosomal associated protein of 25 kDa (SNAP-25) and relatively long-term paralysis. However, nerve-terminal sprouting, which appears to require intact SNAP-25, has been reported to occur much earlier. The difference between the long-term paralysis induced by injection of BoNT/A and the short time needed for sprouting led us to investigate the relationship between BoNT/A catalyzed cleavage of SNAP-25 and muscle function. The effect of BoNT/A on SNAP-25 present in nerve endings innervating gastrocnemius muscles of rats was monitored over time. Cleaved SNAP-25 was found in nerve terminals innervating the muscles within 24 h of inoculation with BoNT/A and was present more than 2 months later. Comparison of the ratios of cleaved to intact SNAP-25 from the onset of BoNT/A-induced paralysis until function was regained indicated that paralysis was probable when the ratio of cleaved to intact SNAP-25 was greater than 0.35.",
author = "Jurasinski, {Christine V.} and Erich Lieth and {Dang Do}, {An N.} and Cara-Lynne Schengrund",
year = "2001",
month = "6",
day = "18",
doi = "10.1016/S0041-0101(01)00082-4",
language = "English (US)",
volume = "39",
pages = "1309--1315",
journal = "Toxicon",
issn = "0041-0101",
publisher = "Elsevier Limited",
number = "9",

}

Correlation of cleavage of SNAP-25 with muscle function in a rat model of Botulinum neurotoxin type A induced paralysis. / Jurasinski, Christine V.; Lieth, Erich; Dang Do, An N.; Schengrund, Cara-Lynne.

In: Toxicon, Vol. 39, No. 9, 18.06.2001, p. 1309-1315.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Correlation of cleavage of SNAP-25 with muscle function in a rat model of Botulinum neurotoxin type A induced paralysis

AU - Jurasinski, Christine V.

AU - Lieth, Erich

AU - Dang Do, An N.

AU - Schengrund, Cara-Lynne

PY - 2001/6/18

Y1 - 2001/6/18

N2 - Injection of botulinum neurotoxin serotype A (BoNT/A) into muscle results in cleavage of the synaptosomal associated protein of 25 kDa (SNAP-25) and relatively long-term paralysis. However, nerve-terminal sprouting, which appears to require intact SNAP-25, has been reported to occur much earlier. The difference between the long-term paralysis induced by injection of BoNT/A and the short time needed for sprouting led us to investigate the relationship between BoNT/A catalyzed cleavage of SNAP-25 and muscle function. The effect of BoNT/A on SNAP-25 present in nerve endings innervating gastrocnemius muscles of rats was monitored over time. Cleaved SNAP-25 was found in nerve terminals innervating the muscles within 24 h of inoculation with BoNT/A and was present more than 2 months later. Comparison of the ratios of cleaved to intact SNAP-25 from the onset of BoNT/A-induced paralysis until function was regained indicated that paralysis was probable when the ratio of cleaved to intact SNAP-25 was greater than 0.35.

AB - Injection of botulinum neurotoxin serotype A (BoNT/A) into muscle results in cleavage of the synaptosomal associated protein of 25 kDa (SNAP-25) and relatively long-term paralysis. However, nerve-terminal sprouting, which appears to require intact SNAP-25, has been reported to occur much earlier. The difference between the long-term paralysis induced by injection of BoNT/A and the short time needed for sprouting led us to investigate the relationship between BoNT/A catalyzed cleavage of SNAP-25 and muscle function. The effect of BoNT/A on SNAP-25 present in nerve endings innervating gastrocnemius muscles of rats was monitored over time. Cleaved SNAP-25 was found in nerve terminals innervating the muscles within 24 h of inoculation with BoNT/A and was present more than 2 months later. Comparison of the ratios of cleaved to intact SNAP-25 from the onset of BoNT/A-induced paralysis until function was regained indicated that paralysis was probable when the ratio of cleaved to intact SNAP-25 was greater than 0.35.

UR - http://www.scopus.com/inward/record.url?scp=0034990815&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034990815&partnerID=8YFLogxK

U2 - 10.1016/S0041-0101(01)00082-4

DO - 10.1016/S0041-0101(01)00082-4

M3 - Article

C2 - 11384718

AN - SCOPUS:0034990815

VL - 39

SP - 1309

EP - 1315

JO - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 9

ER -