Correlation of cytokine elaboration with mononuclear cell adhesion to platelet storage bag plastic polymers: A pilot study

Ikbal Elkattan, James Anderson, J. K. Yun, E. Colton, Roslyn Yomtovian

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The basis for many febrile nonhemolytic transfusion reactions associated with platelet transfusion therapy is cytokine elaboration and accumulation in the storage bag, which correlate with the leukocyte content and the length of platelet storage. We propose that a possible additional variable in the elaboration and accumulation of cytokines is the differential adhesion of mononuclear cells to the plastic substrate of the platelet storage bag. We hypothesize that mononuclear cell adhesion-induced cytokine release is greater in random-donor platelet bags composed of the polyolefin polymer compared to the single-donor apheresis platelet bags composed of the polyvinyl chloride polymer with the tri-(2-ethylhexyl) trimellitate (TEHTM) plasticizer. For four blood donors, we demonstrate preferential mononuclear cell adhesion, in vitro, to discs of polyolefin polymer versus discs of polyvinyl chloride polymer with the TEHTM plasticizer. Scanning electron microscopy corroborates this. In addition, proinflammatory cytokine (interleukin 1β [IL-1β] and tumor necrosis factor alpha [TNF-α]) levels are greater in culture wells containing discs of polyolefin polymer than in those containing discs of polyvinyl chloride polymer with the TEHTM plasticizer, and even more so in storage bags containing polyolefin polymer versus polyvinyl chloride polymer with the TEHTM plasticizer (IL-1β, TNF- α, IL-6, and IL-8). This study suggests, for the first time, that differential plastic substrate mononuclear cell adhesion may contribute to cytokine release during platelet storage. This may represent an additional variable in the pathophysiology of febrile nonhemolytic transfusion reactions in patients receiving stored platelet units.

Original languageEnglish (US)
Pages (from-to)509-513
Number of pages5
JournalClinical and Diagnostic Laboratory Immunology
Volume6
Issue number4
StatePublished - Jul 1 1999

Fingerprint

Cell adhesion
Platelets
Cell Adhesion
Plastics
Polymers
Blood Platelets
Cytokines
Plasticizers
Polyvinyl Chloride
Interleukin-1
Fever
Tumor Necrosis Factor-alpha
Tissue Donors
Platelet Transfusion
Blood Component Removal
Substrates
Blood Donors
Interleukin-8
Electron Scanning Microscopy
Interleukin-6

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

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title = "Correlation of cytokine elaboration with mononuclear cell adhesion to platelet storage bag plastic polymers: A pilot study",
abstract = "The basis for many febrile nonhemolytic transfusion reactions associated with platelet transfusion therapy is cytokine elaboration and accumulation in the storage bag, which correlate with the leukocyte content and the length of platelet storage. We propose that a possible additional variable in the elaboration and accumulation of cytokines is the differential adhesion of mononuclear cells to the plastic substrate of the platelet storage bag. We hypothesize that mononuclear cell adhesion-induced cytokine release is greater in random-donor platelet bags composed of the polyolefin polymer compared to the single-donor apheresis platelet bags composed of the polyvinyl chloride polymer with the tri-(2-ethylhexyl) trimellitate (TEHTM) plasticizer. For four blood donors, we demonstrate preferential mononuclear cell adhesion, in vitro, to discs of polyolefin polymer versus discs of polyvinyl chloride polymer with the TEHTM plasticizer. Scanning electron microscopy corroborates this. In addition, proinflammatory cytokine (interleukin 1β [IL-1β] and tumor necrosis factor alpha [TNF-α]) levels are greater in culture wells containing discs of polyolefin polymer than in those containing discs of polyvinyl chloride polymer with the TEHTM plasticizer, and even more so in storage bags containing polyolefin polymer versus polyvinyl chloride polymer with the TEHTM plasticizer (IL-1β, TNF- α, IL-6, and IL-8). This study suggests, for the first time, that differential plastic substrate mononuclear cell adhesion may contribute to cytokine release during platelet storage. This may represent an additional variable in the pathophysiology of febrile nonhemolytic transfusion reactions in patients receiving stored platelet units.",
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Correlation of cytokine elaboration with mononuclear cell adhesion to platelet storage bag plastic polymers : A pilot study. / Elkattan, Ikbal; Anderson, James; Yun, J. K.; Colton, E.; Yomtovian, Roslyn.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 6, No. 4, 01.07.1999, p. 509-513.

Research output: Contribution to journalArticle

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T1 - Correlation of cytokine elaboration with mononuclear cell adhesion to platelet storage bag plastic polymers

T2 - A pilot study

AU - Elkattan, Ikbal

AU - Anderson, James

AU - Yun, J. K.

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AU - Yomtovian, Roslyn

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